Sensitivity evaluation of a modified real-time reverse-transcription PCR primer to detect a measles virus variant in Japan in 2024

A measles outbreak occurred in Japan in February 2024 due to a measles virus variant that was imported from central Asian countries with three mismatches at the PCR reverse primer (MVN1213R) annealing site. To examine and improve the impact of real-time PCR effectiveness for detecting this variant,...

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Published inJapanese Journal of Infectious Diseases p. JJID.2024.304
Main Authors Morikawa, Saeko, Motomura, Kazushi, Kanbayashi, Daiki, Hiroi, Satoshi, Koyama, Mei, Otsuki, Noriyuki, Kaida, Atsushi, Miyama, Takeshi, Kurata, Takako, Hirai, Yuki
Format Journal Article
LanguageEnglish
Published Japan National Institute of Infectious Diseases 28.02.2025
Subjects
Measles
Real-time PCR
Sensitivity
Variant
Variant
Sensitivity
Real-time PCR
Measles
Online AccessGet full text
ISSN1344-6304
1884-2836
1884-2836
DOI10.7883/yoken.JJID.2024.304

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Abstract A measles outbreak occurred in Japan in February 2024 due to a measles virus variant that was imported from central Asian countries with three mismatches at the PCR reverse primer (MVN1213R) annealing site. To examine and improve the impact of real-time PCR effectiveness for detecting this variant, we compared the sensitivity of real-time PCR between MVN1213R and a modified primer using control RNAs, clinical isolates, and clinical specimens. The median difference in the cycle threshold value was 2.92 (interquartile range, IQR 1.99–3.38) lower using the modified primer compared with MVN1213R. Thus, PCR primer sets should be modified to effectively detect this measles virus mutation.
AbstractList A measles outbreak occurred in Japan in February 2024 due to a measles virus variant that was imported from central Asian countries with three mismatches at the PCR reverse primer (MVN1213R) annealing site. To examine and improve the impact of real-time PCR effectiveness for detecting this variant, we compared the sensitivity of real-time PCR between MVN1213R and a modified primer using control RNAs, clinical isolates, and clinical specimens. The median difference in the cycle threshold value was 2.92 (interquartile range, IQR 1.99-3.38) lower using the modified primer compared with MVN1213R. Thus, PCR primer sets should be modified to effectively detect this measles virus mutation.A measles outbreak occurred in Japan in February 2024 due to a measles virus variant that was imported from central Asian countries with three mismatches at the PCR reverse primer (MVN1213R) annealing site. To examine and improve the impact of real-time PCR effectiveness for detecting this variant, we compared the sensitivity of real-time PCR between MVN1213R and a modified primer using control RNAs, clinical isolates, and clinical specimens. The median difference in the cycle threshold value was 2.92 (interquartile range, IQR 1.99-3.38) lower using the modified primer compared with MVN1213R. Thus, PCR primer sets should be modified to effectively detect this measles virus mutation.
A measles outbreak occurred in Japan in February 2024 due to a measles virus variant that was imported from central Asian countries with three mismatches at the PCR reverse primer (MVN1213R) annealing site. To examine and improve the impact of real-time PCR effectiveness for detecting this variant, we compared the sensitivity of real-time PCR between MVN1213R and a modified primer using control RNAs, clinical isolates, and clinical specimens. The median difference in the cycle threshold value was 2.92 (interquartile range, IQR 1.99-3.38) lower using the modified primer compared with MVN1213R. Thus, PCR primer sets should be modified to effectively detect this measles virus mutation.
ArticleNumber JJID.2024.304
Author Hiroi, Satoshi
Koyama, Mei
Miyama, Takeshi
Motomura, Kazushi
Morikawa, Saeko
Kanbayashi, Daiki
Kaida, Atsushi
Hirai, Yuki
Otsuki, Noriyuki
Kurata, Takako
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  fullname: Morikawa, Saeko
  organization: Division of Microbiology, Osaka Institute of Public Health, Japan
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  fullname: Motomura, Kazushi
  organization: Division of Public Health, Osaka Institute of Public Health, Japan
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  fullname: Kanbayashi, Daiki
  organization: Division of Microbiology, Osaka Institute of Public Health, Japan
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  organization: Division of Microbiology, Osaka Institute of Public Health, Japan
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  fullname: Koyama, Mei
  organization: Division of Microbiology, Osaka Institute of Public Health, Japan
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  fullname: Otsuki, Noriyuki
  organization: Department of Virology, National Institute of Infectious Diseases, Japan
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  fullname: Kaida, Atsushi
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  organization: Division of Microbiology, Osaka Institute of Public Health, Japan
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  fullname: Hirai, Yuki
  organization: Division of Microbiology, Osaka Institute of Public Health, Japan
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10.1016/j.jviromet.2005.10.006
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References_xml – reference: 3. Fappani C, Gori M, Bianchi S, et al. Letter to the editor: further identification of a measles variant displaying mutations impacting molecular diagnostics, Northern Italy, 2024. Euro Surveill. 2024;29:2400079.
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– reference: 2. Pérez-Rodríguez FJ, Cherpillod P, Thomasson V, et al. Identification of a measles variant displaying mutations impacting molecular diagnostics, Geneva, Switzerland, 2023. Euro Surveill. 2024;29:2400034.
– reference: 7. The National Institute of Infectious Diseases. The Manual for Detecting Measles Virus. Available at: https://www.niid.go.jp/niid/images/labmanual/Measles20221003.pdf. Accessed December 21, 2024.
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SubjectTerms Measles
Real-time PCR
Sensitivity
Variant
Title Sensitivity evaluation of a modified real-time reverse-transcription PCR primer to detect a measles virus variant in Japan in 2024
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