High metabolic versatility and phenotypic heterogeneity in a marine non-cyanobacterial diazotroph

• Published in: Current biology Vol. 35; no. 11; pp. 2659 - 2671.e3
• Main Authors: Crétin, Pauline, Mahoudeau, Louise, Joublin-Delavat, Aurélie, Paulhan, Nicolas, Labrune, Elise, Verdon, Julien, Louvet, Isabelle, Maguer, Jean-François, Delavat, François
• Format: Journal Article
• Language: English
• Published: England Elsevier Inc 09.06.2025; Elsevier
• Subjects: Ammonium Compounds - metabolism; Aquatic Organisms; Bacterial Proteins - genetics; Bacterial Proteins - metabolism; bioreporter; HBD; heterotrophic bacterial diazotroph; Life Sciences; marine bacteria; metabolism; N2 fixation; NCD; nitrogen; Nitrogen - metabolism; Nitrogen Fixation - physiology; Nitrogenase - genetics; Nitrogenase - metabolism; non-cyanobacterial diazotroph; Phenotype; phenotypic heterogeneity; Seawater - microbiology; single-cell approaches; Vibrio; Vibrio - genetics; Vibrio - growth & development; Vibrio - metabolism; Vibrio - physiology; heterotrophic bacterial diazotroph; N2 fixation; non-cyanobacterial diazotroph; nitrogen; phenotypic heterogeneity; marine bacteria; metabolism; bioreporter; HBD; single-cell approaches; NCD; Vibrio; N fixation

Marine non-cyanobacterial diazotrophs (NCDs) are widespread in the oceans, but the processes controlling nitrogen fixation in cell populations remain understudied. In this study, we combined high-throughput sequencing, genetic and physiological characterization, and single-cell quantification of nit...