The use of a cationic liposome formulation (DOTAP) mixed with a recombinant tumor-associated antigen to induce immune responses and protective immunity in mice

• Published in: Journal of immunotherapy (1997) Vol. 21; no. 3; p. 159
• Main Authors: Bei, R, Guptill, V, Masuelli, L, Kashmiri, S V, Muraro, R, Frati, L, Schlom, J, Kantor, J
• Format: Journal Article
• Language: English
• Published: United States 01.05.1998
• Subjects: Animals; Antigens - immunology; Baculoviridae - genetics; Carcinoembryonic Antigen - genetics; Carcinoembryonic Antigen - immunology; Cell Line; Fatty Acids, Monounsaturated - immunology; Fluorescent Dyes; Humans; Immunization; Immunoglobulin G - blood; Mice; Mice, Inbred BALB C; Mice, Inbred C57BL; Neoplasm Transplantation; Neoplasms, Experimental - prevention & control; Quaternary Ammonium Compounds - immunology; Recombinant Proteins

The cationic liposome DOTAP is a well-known transfection reagent. It has been manufactured and approved for clinical use, is readily available, and can be easily used as an adjuvant. These characteristics prompted us to investigate the effectiveness of DOTAP as an adjuvant to induce immune responses...