Intracellular Compartmentalization of PDE4 Cyclic AMP-Specific Phosphodiesterases
The PDE4 cyclic AMP-specific phosphodiesterase family comprises a large number of different isoforms encoded by four distinct genes, with additional complexity arising through alternate mRNA splicing. This generates a number of distinct PDE4 isoforms with unique N-terminal regions. The range of such...
Saved in:
Published in | Methods (San Diego, Calif.) Vol. 14; no. 1; pp. 65 - 79 |
---|---|
Main Authors | , , , , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
United States
Elsevier Inc
01.01.1998
|
Subjects | |
Online Access | Get full text |
Cover
Loading…
Abstract | The PDE4 cyclic AMP-specific phosphodiesterase family comprises a large number of different isoforms encoded by four distinct genes, with additional complexity arising through alternate mRNA splicing. This generates a number of distinct PDE4 isoforms with unique N-terminal regions. The range of such splice variants emanating from the four PDE4 genes appears to be highly conserved across species. One key role for such regions appears to be their potential to target isoforms to specific intracellular sites. Evidence for such a targeting role for these N-terminal regions can be gleaned by a variety of techniques. These include subcellular fractionation, confocal microscopy, binding assays to show association with proteins havingsrchomology 3 (SH3) domains, and generation of chimeric constructs of these N-terminal regions with proteins that are normally expressed in the cytosol. |
---|---|
AbstractList | The PDE4 cyclic AMP-specific phosphodiesterase family comprises a large number of different isoforms encoded by four distinct genes, with additional complexity arising through alternate mRNA splicing. This generates a number of distinct PDE4 isoforms with unique N-terminal regions. The range of such splice variants emanating from the four PDE4 genes appears to be highly conserved across species. One key role for such regions appears to be their potential to target isoforms to specific intracellular sites. Evidence for such a targeting role for these N-terminal regions can be gleaned by a variety of techniques. These include subcellular fractionation, confocal microscopy, binding assays to show association with proteins having src homology 3 (SH3) domains, and generation of chimeric constructs of these N-terminal regions with proteins that are normally expressed in the cytosol. The PDE4 cyclic AMP-specific phosphodiesterase family comprises a large number of different isoforms encoded by four distinct genes, with additional complexity arising through alternate mRNA splicing. This generates a number of distinct PDE4 isoforms with unique N-terminal regions. The range of such splice variants emanating from the four PDE4 genes appears to be highly conserved across species. One key role for such regions appears to be their potential to target isoforms to specific intracellular sites. Evidence for such a targeting role for these N-terminal regions can be gleaned by a variety of techniques. These include subcellular fractionation, confocal microscopy, binding assays to show association with proteins havingsrchomology 3 (SH3) domains, and generation of chimeric constructs of these N-terminal regions with proteins that are normally expressed in the cytosol. |
Author | Beard, M. MacKenzie, S.J. Scotland, G. Rena, N.G. Peden, A.H. Ross, A.H. McCallum, F. Erdogan, S. Pooley, L. Houslay, M.D. Yarwood, S.J. Huston, E. |
Author_xml | – sequence: 1 givenname: G. surname: Scotland fullname: Scotland, G. – sequence: 2 givenname: M. surname: Beard fullname: Beard, M. – sequence: 3 givenname: S. surname: Erdogan fullname: Erdogan, S. – sequence: 4 givenname: E. surname: Huston fullname: Huston, E. – sequence: 5 givenname: F. surname: McCallum fullname: McCallum, F. – sequence: 6 givenname: S.J. surname: MacKenzie fullname: MacKenzie, S.J. – sequence: 7 givenname: A.H. surname: Peden fullname: Peden, A.H. – sequence: 8 givenname: L. surname: Pooley fullname: Pooley, L. – sequence: 9 givenname: N.G. surname: Rena fullname: Rena, N.G. – sequence: 10 givenname: A.H. surname: Ross fullname: Ross, A.H. – sequence: 11 givenname: S.J. surname: Yarwood fullname: Yarwood, S.J. – sequence: 12 givenname: M.D. surname: Houslay fullname: Houslay, M.D. |
BackLink | https://www.ncbi.nlm.nih.gov/pubmed/9500859$$D View this record in MEDLINE/PubMed |
BookMark | eNp1kFFLwzAUhYNM5jZ99U3oH2hNmjRtHsecOpg4UZ9Dmt6wSNuUpBPmr7dlwzef7j2cew6Xb44mrWsBoVuCE4Ixv2-g3ydEiDzBGecXaEawyGJBKJ6MO-NxilN6heYhfGGMSZoXUzQVGcZFJmbobdP2Xmmo60OtfLRyTad830Dbq9r-qN66NnIm2j2sWbQ66trqaPmyi9870NYMYrd3odu7ykLowasA4RpdGlUHuDnPBfp8XH-snuPt69NmtdzGmjLSxyznFWfUMAoYsCp4CUbkKc51XpYspyzjmSmLiiiRacqrURJGqRri1ChDFyg59WrvQvBgZOdto_xREixHNHJEI0c0ckQzBO5Oge5QNlD9nZ9ZDH5x8mH4-tuCl0FbaDVU1oPuZeXsf9W_YNJ1Bw |
CitedBy_id | crossref_primary_10_1046_j_1432_1327_1999_00418_x crossref_primary_10_1016_S0165_3806_98_00150_3 crossref_primary_10_1165_rcmb_2002_0247OC crossref_primary_10_1007_s00383_013_3460_z crossref_primary_10_1038_sj_bjp_0702911 crossref_primary_10_1007_s11427_021_1975_9 crossref_primary_10_1152_ajprenal_00079_2004 crossref_primary_10_1038_mp_a000104_01 |
Cites_doi | 10.1042/bj3080683 10.1042/bj3080673 10.1042/bst0230592 10.1016/S1054-3589(08)60128-3 10.1016/S0021-9258(17)32297-4 10.1016/0092-8674(92)90504-6 10.1210/edrv-16-3-370 10.1042/bj2920677 10.1042/bj3280549 10.1074/jbc.271.49.31334 10.1074/jbc.271.28.16526 10.1016/S0021-9258(19)37050-4 10.1042/bj3180255 10.1042/bj3040399 10.1016/0898-6568(95)02003-9 10.1042/bj3280539 10.1074/jbc.271.2.1065 10.1006/jmcc.1997.0544 10.1074/jbc.271.28.16703 10.1042/bj3210177 10.1016/S0968-0004(97)01050-5 10.1042/bj3060801 10.1042/bj3100965 10.1128/MCB.13.10.6558 10.1042/bst0240980 10.1042/bst0250374 10.1016/0968-0004(90)90303-S 10.1042/bj3210165 10.1016/0962-8924(93)90194-6 10.1016/0898-6568(94)90018-3 |
ContentType | Journal Article |
Copyright | 1998 Academic Press Copyright 1998 Academic Press. |
Copyright_xml | – notice: 1998 Academic Press – notice: Copyright 1998 Academic Press. |
DBID | CGR CUY CVF ECM EIF NPM AAYXX CITATION |
DOI | 10.1006/meth.1997.0566 |
DatabaseName | Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed CrossRef |
DatabaseTitle | MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) CrossRef |
DatabaseTitleList | MEDLINE |
Database_xml | – sequence: 1 dbid: NPM name: PubMed url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 2 dbid: EIF name: MEDLINE url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search sourceTypes: Index Database |
DeliveryMethod | fulltext_linktorsrc |
Discipline | Anatomy & Physiology Chemistry |
EISSN | 1095-9130 |
EndPage | 79 |
ExternalDocumentID | 10_1006_meth_1997_0566 9500859 S1046202397905661 |
Genre | Research Support, Non-U.S. Gov't Journal Article |
GroupedDBID | --- --K --M -~X .GJ .~1 0R~ 123 1B1 1RT 1~. 1~5 29M 4.4 457 4G. 53G 5VS 7-5 71M 8P~ 9JM AABNK AACTN AAEDT AAEDW AAIAV AAIKJ AAKOC AALRI AAOAW AAQFI AAQXK AAXUO ABEFU ABFNM ABFRF ABGSF ABJNI ABMAC ABUDA ABXDB ABYKQ ACDAQ ACGFO ACGFS ACRLP ADBBV ADEZE ADFGL ADMUD ADUVX AEBSH AEFWE AEHWI AEKER AENEX AFKWA AFTJW AFXIZ AGHFR AGRDE AGUBO AGYEJ AHHHB AIEXJ AIKHN AITUG AJBFU AJOXV ALMA_UNASSIGNED_HOLDINGS AMFUW AMRAJ ASPBG AVWKF AXJTR AZFZN BKOJK BLXMC CAG COF CS3 DM4 DOVZS DU5 EBS EFBJH EFLBG EJD EO8 EO9 EP2 EP3 F5P FDB FEDTE FGOYB FIRID FNPLU FYGXN G-2 G-Q GBLVA HLW HMG HVGLF HZ~ IHE J1W K-O KOM LG5 LX2 LZ5 M41 MO0 N9A O-L O9- OAUVE OZT P-8 P-9 P2P PC. Q38 R2- RIG ROL RPZ SBG SCC SDF SDG SDP SES SEW SIN SPCBC SSU SSZ T5K WUQ XPP Y6R ZGI ZMT ZU3 ~G- AAHBH AAXKI AFJKZ AKRWK CGR CUY CVF ECM EIF NPM AAYXX CITATION |
ID | FETCH-LOGICAL-c341t-476d643f43e0e0a86bef97207c7bb4734565fb8d1a95c36d565f1433ac343faf3 |
IEDL.DBID | AIKHN |
ISSN | 1046-2023 |
IngestDate | Thu Sep 26 18:28:17 EDT 2024 Sat Sep 28 07:41:22 EDT 2024 Fri Feb 23 02:32:39 EST 2024 |
IsPeerReviewed | true |
IsScholarly | true |
Issue | 1 |
Language | English |
License | Copyright 1998 Academic Press. |
LinkModel | DirectLink |
MergedId | FETCHMERGED-LOGICAL-c341t-476d643f43e0e0a86bef97207c7bb4734565fb8d1a95c36d565f1433ac343faf3 |
PMID | 9500859 |
PageCount | 15 |
ParticipantIDs | crossref_primary_10_1006_meth_1997_0566 pubmed_primary_9500859 elsevier_sciencedirect_doi_10_1006_meth_1997_0566 |
PublicationCentury | 1900 |
PublicationDate | January 1998 1998-Jan 1998-01-00 |
PublicationDateYYYYMMDD | 1998-01-01 |
PublicationDate_xml | – month: 01 year: 1998 text: January 1998 |
PublicationDecade | 1990 |
PublicationPlace | United States |
PublicationPlace_xml | – name: United States |
PublicationTitle | Methods (San Diego, Calif.) |
PublicationTitleAlternate | Methods |
PublicationYear | 1998 |
Publisher | Elsevier Inc |
Publisher_xml | – name: Elsevier Inc |
References | Sette, Conti (ME970566RF17) 1996; 271 Houslay, Milligan (ME970566RF20) 1997; 22 Pawson, Gish (ME970566RF27) 1992; 71 Shakur, Pryde, Houslay (ME970566RF13) 1993; 292 Pelham (ME970566RF25) 1990; 15 Erdogan, Houslay (ME970566RF10) 1997; 321 Owens, Catterall, Batty, Jappy, Russell, Smith, O'Connell, Perry (ME970566RF33) 1997 Scotland, Houslay (ME970566RF14) 1995; 308 Bolger, McPhee, Houslay (ME970566RF8) 1996; 271 Smith, Scotland, Beattie, Trayer, Houslay (ME970566RF19) 1996; 271 Bolger (ME970566RF1) 1994; 6 McPhee, Pooley, Lobban, Bolger, Houslay (ME970566RF5) 1995; 310 Houslay (ME970566RF24) 1996; 24 Mayer, Baltimore (ME970566RF26) 1993; 3 Lobban, Shakur, Beattie, Houslay (ME970566RF6) 1994; 304 Shakur, Wilson, Pooley, Lobban, Griffiths, Campbell, Beattie, Daly, Houslay (ME970566RF4) 1995; 306 Conti, Nemoz, Sette, Vicini (ME970566RF2) 1995; 16 Bolger, Michaeli, Martins, St John, Steiner, Rodgers, Riggs, Wigler, Ferguson (ME970566RF34) 1993; 13 Bolger, Erdogan, Jones, Loughney, Wilkinson, Farrell, Houslay (ME970566RF9) 1997; 328 Huston, Lumb, Russell, Catterall, Ross, Steele, Bolger, Perry, Owens, Houslay (ME970566RF7) 1997; 328 Coghlan, Hausken, Scott (ME970566RF22) 1995; 23 Klauck, Scott (ME970566RF21) 1995; 7 Jacobitz, McLaughlin, Livi, Burman, Torphy (ME970566RF31) 1996; 50 Pooley, Shakur, Rena, Houslay (ME970566RF15) 1997; 271 Jacobitz, Ryan, McLaughlin, Livi, DeWolf, Torphy (ME970566RF30) 1997; 50 Sette, Vicini, Conti (ME970566RF18) 1994; 269 O'Connell, McCallum, McPhee, Wakefield, Houslay, Wishart, Bolger, Frame, Houslay (ME970566RF12) 1996; 318 Houslay, Sullivan, Bolger (ME970566RF3) 1998; 44 Kostic, Erdogan, Rena, Borchert, Hoch, Bartel, Scotland, Huston, Houslay, Krause (ME970566RF11) 1997; 29 Horton, Sullivan, Houslay (ME970566RF32) 1995; 308 Huston, Pooley, Julien, Scotland, McPhee, Sullivan, Bolger, Houslay (ME970566RF16) 1996; 271 Houslay, Scotland, Erdogan, Huston, Mackenzie, McCallum, McPhee, Pooley, Rena, Ross, Beard, Peder, Begg, Wilkinson, Yarwood, Ackerman, Houslay, Hoffman, Engels, Sullivan, Bolger (ME970566RF23) 1997; 25 Charbonneau (ME970566RF28) 1990 Jin, Swinnen, Conti (ME970566RF29) 1992; 267 Bolger (10.1006/meth.1997.0566_ME970566RF8) 1996; 271 Jin (10.1006/meth.1997.0566_ME970566RF29) 1992; 267 Huston (10.1006/meth.1997.0566_ME970566RF7) 1997; 328 Erdogan (10.1006/meth.1997.0566_ME970566RF10) 1997; 321 Shakur (10.1006/meth.1997.0566_ME970566RF4) 1995; 306 Houslay (10.1006/meth.1997.0566_ME970566RF20) 1997; 22 Jacobitz (10.1006/meth.1997.0566_ME970566RF30) 1997; 50 Bolger (10.1006/meth.1997.0566_ME970566RF9) 1997; 328 Mayer (10.1006/meth.1997.0566_ME970566RF26) 1993; 3 Owens (10.1006/meth.1997.0566_ME970566RF33) 1997 Klauck (10.1006/meth.1997.0566_ME970566RF21) 1995; 7 Bolger (10.1006/meth.1997.0566_ME970566RF1) 1994; 6 Conti (10.1006/meth.1997.0566_ME970566RF2) 1995; 16 Huston (10.1006/meth.1997.0566_ME970566RF16) 1996; 271 Pawson (10.1006/meth.1997.0566_ME970566RF27) 1992; 71 Sette (10.1006/meth.1997.0566_ME970566RF18) 1994; 269 Houslay (10.1006/meth.1997.0566_ME970566RF24) 1996; 24 Horton (10.1006/meth.1997.0566_ME970566RF32) 1995; 308 Bolger (10.1006/meth.1997.0566_ME970566RF34) 1993; 13 Pooley (10.1006/meth.1997.0566_ME970566RF15) 1997; 271 Jacobitz (10.1006/meth.1997.0566_ME970566RF31) 1996; 50 Houslay (10.1006/meth.1997.0566_ME970566RF3) 1998; 44 Shakur (10.1006/meth.1997.0566_ME970566RF13) 1993; 292 Charbonneau (10.1006/meth.1997.0566_ME970566RF28) 1990 Pelham (10.1006/meth.1997.0566_ME970566RF25) 1990; 15 Smith (10.1006/meth.1997.0566_ME970566RF19) 1996; 271 Coghlan (10.1006/meth.1997.0566_ME970566RF22) 1995; 23 McPhee (10.1006/meth.1997.0566_ME970566RF5) 1995; 310 Kostic (10.1006/meth.1997.0566_ME970566RF11) 1997; 29 Sette (10.1006/meth.1997.0566_ME970566RF17) 1996; 271 Scotland (10.1006/meth.1997.0566_ME970566RF14) 1995; 308 O'Connell (10.1006/meth.1997.0566_ME970566RF12) 1996; 318 Houslay (10.1006/meth.1997.0566_ME970566RF23) 1997; 25 Lobban (10.1006/meth.1997.0566_ME970566RF6) 1994; 304 |
References_xml | – volume: 304 start-page: 399 year: 1994 end-page: 406 ident: ME970566RF6 publication-title: Biochem. J. contributor: fullname: Houslay – volume: 318 start-page: 255 year: 1996 end-page: 262 ident: ME970566RF12 publication-title: Biochem. J. contributor: fullname: Houslay – volume: 271 start-page: 16526 year: 1996 end-page: 16534 ident: ME970566RF17 publication-title: J. Biol. Chem. contributor: fullname: Conti – volume: 71 start-page: 359 year: 1992 end-page: 362 ident: ME970566RF27 publication-title: Cell contributor: fullname: Gish – volume: 328 start-page: 549 year: 1997 end-page: 558 ident: ME970566RF7 publication-title: Biochem. J. contributor: fullname: Houslay – volume: 50 start-page: 891 year: 1996 end-page: 899 ident: ME970566RF31 publication-title: Mol. Pharmacol. contributor: fullname: Torphy – volume: 328 start-page: 539 year: 1997 end-page: 548 ident: ME970566RF9 publication-title: Biochem. J. contributor: fullname: Houslay – volume: 271 start-page: 31334 year: 1996 end-page: 31344 ident: ME970566RF16 publication-title: J. Biol. Chem. contributor: fullname: Houslay – volume: 271 start-page: 16703 year: 1996 end-page: 16711 ident: ME970566RF19 publication-title: J. Biol. Chem. contributor: fullname: Houslay – volume: 23 start-page: 592 year: 1995 end-page: 596 ident: ME970566RF22 publication-title: Biochem. Soc. Trans. contributor: fullname: Scott – volume: 306 start-page: 801 year: 1995 end-page: 809 ident: ME970566RF4 publication-title: Biochem. J. contributor: fullname: Houslay – volume: 7 start-page: 747 year: 1995 end-page: 757 ident: ME970566RF21 publication-title: Cell. Signal. contributor: fullname: Scott – volume: 15 start-page: 483 year: 1990 end-page: 486 ident: ME970566RF25 publication-title: Trends Biochem. Sci. contributor: fullname: Pelham – volume: 269 start-page: 18271 year: 1994 end-page: 18274 ident: ME970566RF18 publication-title: J. Biol. Chem. contributor: fullname: Conti – volume: 271 start-page: 1065 year: 1996 end-page: 1071 ident: ME970566RF8 publication-title: J. Biol. Chem. contributor: fullname: Houslay – volume: 16 start-page: 370 year: 1995 end-page: 389 ident: ME970566RF2 publication-title: Endocrine Rev. contributor: fullname: Vicini – year: 1990 ident: ME970566RF28 publication-title: Cyclic Nucleotide Phosphodiesterases contributor: fullname: Charbonneau – volume: 308 start-page: 683 year: 1995 end-page: 691 ident: ME970566RF32 publication-title: Biochem. J. contributor: fullname: Houslay – volume: 310 start-page: 965 year: 1995 end-page: 974 ident: ME970566RF5 publication-title: Biochem. J. contributor: fullname: Houslay – volume: 24 start-page: 980 year: 1996 end-page: 986 ident: ME970566RF24 publication-title: Biochem. Soc. Trans. contributor: fullname: Houslay – volume: 3 start-page: 8 year: 1993 end-page: 13 ident: ME970566RF26 publication-title: Trends Cell Biol. contributor: fullname: Baltimore – volume: 292 start-page: 677 year: 1993 end-page: 686 ident: ME970566RF13 publication-title: Biochem. J. contributor: fullname: Houslay – volume: 308 start-page: 673 year: 1995 end-page: 681 ident: ME970566RF14 publication-title: Biochem. J. contributor: fullname: Houslay – volume: 13 start-page: 6558 year: 1993 end-page: 6571 ident: ME970566RF34 publication-title: Mol. Cell. Biol. contributor: fullname: Ferguson – volume: 22 start-page: 217 year: 1997 end-page: 224 ident: ME970566RF20 publication-title: Trends Biochem. Sci. contributor: fullname: Milligan – volume: 271 start-page: 177 year: 1997 end-page: 185 ident: ME970566RF15 publication-title: Biochem. J. contributor: fullname: Houslay – volume: 29 start-page: 3135 year: 1997 end-page: 3146 ident: ME970566RF11 publication-title: J. Mol. Cell Cardiol. contributor: fullname: Krause – volume: 267 start-page: 18929 year: 1992 end-page: 18939 ident: ME970566RF29 publication-title: J. Biol. Chem. contributor: fullname: Conti – volume: 44 start-page: 225 year: 1998 end-page: 342 ident: ME970566RF3 publication-title: Adv. Pharmacol. contributor: fullname: Bolger – volume: 25 start-page: 374 year: 1997 end-page: 381 ident: ME970566RF23 publication-title: Biochem. Soc. Trans. contributor: fullname: Bolger – volume: 321 start-page: 165 year: 1997 end-page: 175 ident: ME970566RF10 publication-title: Biochem. J. contributor: fullname: Houslay – year: 1997 ident: ME970566RF33 publication-title: Biochem. J. contributor: fullname: Perry – volume: 50 start-page: 891 year: 1997 end-page: 899 ident: ME970566RF30 publication-title: Mol. Pharmacol. contributor: fullname: Torphy – volume: 6 start-page: 851 year: 1994 end-page: 859 ident: ME970566RF1 publication-title: Cell. Signal. contributor: fullname: Bolger – volume: 308 start-page: 683 year: 1995 ident: 10.1006/meth.1997.0566_ME970566RF32 publication-title: Biochem. J. doi: 10.1042/bj3080683 contributor: fullname: Horton – volume: 308 start-page: 673 year: 1995 ident: 10.1006/meth.1997.0566_ME970566RF14 publication-title: Biochem. J. doi: 10.1042/bj3080673 contributor: fullname: Scotland – volume: 23 start-page: 592 year: 1995 ident: 10.1006/meth.1997.0566_ME970566RF22 publication-title: Biochem. Soc. Trans. doi: 10.1042/bst0230592 contributor: fullname: Coghlan – volume: 44 start-page: 225 year: 1998 ident: 10.1006/meth.1997.0566_ME970566RF3 publication-title: Adv. Pharmacol. doi: 10.1016/S1054-3589(08)60128-3 contributor: fullname: Houslay – volume: 269 start-page: 18271 year: 1994 ident: 10.1006/meth.1997.0566_ME970566RF18 publication-title: J. Biol. Chem. doi: 10.1016/S0021-9258(17)32297-4 contributor: fullname: Sette – year: 1997 ident: 10.1006/meth.1997.0566_ME970566RF33 publication-title: Biochem. J. contributor: fullname: Owens – volume: 71 start-page: 359 year: 1992 ident: 10.1006/meth.1997.0566_ME970566RF27 publication-title: Cell doi: 10.1016/0092-8674(92)90504-6 contributor: fullname: Pawson – volume: 16 start-page: 370 year: 1995 ident: 10.1006/meth.1997.0566_ME970566RF2 publication-title: Endocrine Rev. doi: 10.1210/edrv-16-3-370 contributor: fullname: Conti – volume: 292 start-page: 677 year: 1993 ident: 10.1006/meth.1997.0566_ME970566RF13 publication-title: Biochem. J. doi: 10.1042/bj2920677 contributor: fullname: Shakur – volume: 328 start-page: 549 year: 1997 ident: 10.1006/meth.1997.0566_ME970566RF7 publication-title: Biochem. J. doi: 10.1042/bj3280549 contributor: fullname: Huston – volume: 271 start-page: 31334 year: 1996 ident: 10.1006/meth.1997.0566_ME970566RF16 publication-title: J. Biol. Chem. doi: 10.1074/jbc.271.49.31334 contributor: fullname: Huston – volume: 271 start-page: 16526 year: 1996 ident: 10.1006/meth.1997.0566_ME970566RF17 publication-title: J. Biol. Chem. doi: 10.1074/jbc.271.28.16526 contributor: fullname: Sette – volume: 267 start-page: 18929 year: 1992 ident: 10.1006/meth.1997.0566_ME970566RF29 publication-title: J. Biol. Chem. doi: 10.1016/S0021-9258(19)37050-4 contributor: fullname: Jin – volume: 318 start-page: 255 year: 1996 ident: 10.1006/meth.1997.0566_ME970566RF12 publication-title: Biochem. J. doi: 10.1042/bj3180255 contributor: fullname: O'Connell – volume: 304 start-page: 399 year: 1994 ident: 10.1006/meth.1997.0566_ME970566RF6 publication-title: Biochem. J. doi: 10.1042/bj3040399 contributor: fullname: Lobban – volume: 7 start-page: 747 year: 1995 ident: 10.1006/meth.1997.0566_ME970566RF21 publication-title: Cell. Signal. doi: 10.1016/0898-6568(95)02003-9 contributor: fullname: Klauck – volume: 328 start-page: 539 year: 1997 ident: 10.1006/meth.1997.0566_ME970566RF9 publication-title: Biochem. J. doi: 10.1042/bj3280539 contributor: fullname: Bolger – volume: 271 start-page: 1065 year: 1996 ident: 10.1006/meth.1997.0566_ME970566RF8 publication-title: J. Biol. Chem. doi: 10.1074/jbc.271.2.1065 contributor: fullname: Bolger – year: 1990 ident: 10.1006/meth.1997.0566_ME970566RF28 contributor: fullname: Charbonneau – volume: 29 start-page: 3135 year: 1997 ident: 10.1006/meth.1997.0566_ME970566RF11 publication-title: J. Mol. Cell Cardiol. doi: 10.1006/jmcc.1997.0544 contributor: fullname: Kostic – volume: 271 start-page: 16703 year: 1996 ident: 10.1006/meth.1997.0566_ME970566RF19 publication-title: J. Biol. Chem. doi: 10.1074/jbc.271.28.16703 contributor: fullname: Smith – volume: 271 start-page: 177 year: 1997 ident: 10.1006/meth.1997.0566_ME970566RF15 publication-title: Biochem. J. doi: 10.1042/bj3210177 contributor: fullname: Pooley – volume: 22 start-page: 217 year: 1997 ident: 10.1006/meth.1997.0566_ME970566RF20 publication-title: Trends Biochem. Sci. doi: 10.1016/S0968-0004(97)01050-5 contributor: fullname: Houslay – volume: 50 start-page: 891 year: 1996 ident: 10.1006/meth.1997.0566_ME970566RF31 publication-title: Mol. Pharmacol. contributor: fullname: Jacobitz – volume: 306 start-page: 801 year: 1995 ident: 10.1006/meth.1997.0566_ME970566RF4 publication-title: Biochem. J. doi: 10.1042/bj3060801 contributor: fullname: Shakur – volume: 310 start-page: 965 year: 1995 ident: 10.1006/meth.1997.0566_ME970566RF5 publication-title: Biochem. J. doi: 10.1042/bj3100965 contributor: fullname: McPhee – volume: 50 start-page: 891 year: 1997 ident: 10.1006/meth.1997.0566_ME970566RF30 publication-title: Mol. Pharmacol. contributor: fullname: Jacobitz – volume: 13 start-page: 6558 year: 1993 ident: 10.1006/meth.1997.0566_ME970566RF34 publication-title: Mol. Cell. Biol. doi: 10.1128/MCB.13.10.6558 contributor: fullname: Bolger – volume: 24 start-page: 980 year: 1996 ident: 10.1006/meth.1997.0566_ME970566RF24 publication-title: Biochem. Soc. Trans. doi: 10.1042/bst0240980 contributor: fullname: Houslay – volume: 25 start-page: 374 year: 1997 ident: 10.1006/meth.1997.0566_ME970566RF23 publication-title: Biochem. Soc. Trans. doi: 10.1042/bst0250374 contributor: fullname: Houslay – volume: 15 start-page: 483 year: 1990 ident: 10.1006/meth.1997.0566_ME970566RF25 publication-title: Trends Biochem. Sci. doi: 10.1016/0968-0004(90)90303-S contributor: fullname: Pelham – volume: 321 start-page: 165 year: 1997 ident: 10.1006/meth.1997.0566_ME970566RF10 publication-title: Biochem. J. doi: 10.1042/bj3210165 contributor: fullname: Erdogan – volume: 3 start-page: 8 year: 1993 ident: 10.1006/meth.1997.0566_ME970566RF26 publication-title: Trends Cell Biol. doi: 10.1016/0962-8924(93)90194-6 contributor: fullname: Mayer – volume: 6 start-page: 851 year: 1994 ident: 10.1006/meth.1997.0566_ME970566RF1 publication-title: Cell. Signal. doi: 10.1016/0898-6568(94)90018-3 contributor: fullname: Bolger |
SSID | ssj0001278 |
Score | 1.616349 |
Snippet | The PDE4 cyclic AMP-specific phosphodiesterase family comprises a large number of different isoforms encoded by four distinct genes, with additional complexity... |
SourceID | crossref pubmed elsevier |
SourceType | Aggregation Database Index Database Publisher |
StartPage | 65 |
SubjectTerms | 3',5'-Cyclic-AMP Phosphodiesterases - analysis Alternative Splicing - genetics Animals COS Cells Cyclic Nucleotide Phosphodiesterases, Type 4 DNA Primers - chemistry Fluorescent Antibody Technique Gene Expression - genetics Isoenzymes - analysis Membrane Proteins - analysis Microscopy, Confocal Mutagenesis, Site-Directed - genetics Plasmids - genetics Protein Biosynthesis - genetics Recombinant Fusion Proteins - genetics Recombinant Fusion Proteins - isolation & purification Sequence Deletion src Homology Domains - genetics Transcription, Genetic - genetics Transfection - genetics |
Title | Intracellular Compartmentalization of PDE4 Cyclic AMP-Specific Phosphodiesterases |
URI | https://dx.doi.org/10.1006/meth.1997.0566 https://www.ncbi.nlm.nih.gov/pubmed/9500859 |
Volume | 14 |
hasFullText | 1 |
inHoldings | 1 |
isFullTextHit | |
isPrint | |
link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV07T8MwED6VdoAFQUtFeVQeEEwhaeI4yRiFVi2IqggqdYvixBaVIKnaMHTht-PLo4IBBkZHsmWd7bvvLnffAVypO2snMuLqfUumUWopPRg7ppYknpGY1DV5XLB9Ttl4Tu8X9qIBQV0Lg2mVle4vdXqhrasveiVNfbVc6s_4dxKbf3vIMcXQBWopc0RpE1r-5GE83SnkgemUFXGUaTih5m40mI5tmrFgD-k7C6LEv2zTN8MzOoLDCjESv9zUMTRE2oaOnypv-X1LrkmRw1kEx9uwH9T92zrwNMG4LQbmMdOUFA9_nZdc_lXxJckkmd0NKQm28dsyJv7jTCsa0ks1mL1mm9VrhlmGWKW8EZsTmI-GL8FYqxooaLEyTrlGHZYoxCGpJQxhRC7jQnqOaTixwzl1LERzkrvJIPLs2GIJDhV-siI13ZKRtLrQTLNUnAJxFA6y3ZgVLpgwbB7xiBqJOn8ZmZ5gPbipRReuSp6MsGREZiEKOUQhhyjkHgxqyYY_TjpUSvzXOd3yCHZrezaCRe_sH4udw0FZWIhxlAto5usPcamQRc77sHf7OehX9-cLcCfMrA |
link.rule.ids | 315,786,790,4521,24144,27957,27958,45620,45714 |
linkProvider | Elsevier |
linkToHtml | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV07b8IwED4hGOhStVBU-vRQtVNESBw7GSMKgvIQVUFii-LEFkgtQUAH_n19eaB2aIeOjnSWdWefP1_uvgN40HvWiVUo9PlWzKDU1n4w4pYRx54ZW9S1RJSyfU5Yf05fFs6iBJ2iFgbTKnPfn_n01FvnX1q5Nlub1ar1hn8nsfm3hxxTDJ9AFerwNi1DxR8M-5OjQ25bPKuIo8xAgYK70WQtbNOMBXtI35kSJf51N327eHpncJojRuJnizqHklzXoO6v9Wv540AeSZrDmQbHa1DtFP3b6vA6wLgtBuYx05SkB3-7z7j88-JLkigyfe5S0jlE76uI-OOpkTakV3owXSa7zTLBLEOsUt7J3QXMe91Zp2_kDRSMSF9Oe4NyFmvEoagtTWmGLhNSedwyecSFoNxGNKeEG7dDz4lsFuNQ4yc71OK2CpXdgPI6WctLIFzjIMeNWPoEk6YjQhFSM9b2V6HlSdaEp0J1wSbjyQgyRmQWoJIDVHKASm5Cu9Bs8MPSgXbiv8o0MhMc5_YcBIve1T8mu4dqfzYeBaPBZHgNJ1mRIcZUbqC8337KW40y9uIu30VfYp_Ong |
openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Intracellular+Compartmentalization+of+PDE4+Cyclic+AMP-Specific+Phosphodiesterases&rft.jtitle=Methods+%28San+Diego%2C+Calif.%29&rft.au=Scotland%2C+G.&rft.au=Beard%2C+M.&rft.au=Erdogan%2C+S.&rft.au=Huston%2C+E.&rft.date=1998-01-01&rft.issn=1046-2023&rft.volume=14&rft.issue=1&rft.spage=65&rft.epage=79&rft_id=info:doi/10.1006%2Fmeth.1997.0566&rft.externalDBID=n%2Fa&rft.externalDocID=10_1006_meth_1997_0566 |
thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=1046-2023&client=summon |
thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=1046-2023&client=summon |
thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=1046-2023&client=summon |