Comprehensive Immunophenotyping of Cerebrospinal Fluid Cells in Patients with Neuroimmunological Diseases

• Published in: The Journal of immunology (1950) Vol. 192; no. 6; pp. 2551 - 2563
• Main Authors: Han, Sungpil, Lin, Yen Chih, Wu, Tianxia, Salgado, Alan D, Mexhitaj, Ina, Wuest, Simone C, Romm, Elena, Ohayon, Joan, Goldbach-Mansky, Raphaela, Vanderver, Adeline, Marques, Adriana, Toro, Camilo, Williamson, Peter, Cortese, Irene, Bielekova, Bibiana
• Format: Journal Article
• Language: English
• Published: England 15.03.2014
• Subjects: Adolescent; Adult; Aged; Autoimmune Diseases of the Nervous System - blood; Autoimmune Diseases of the Nervous System - cerebrospinal fluid; Autoimmune Diseases of the Nervous System - immunology; CD56 Antigen - immunology; CD56 Antigen - metabolism; Cell Count; Dendritic Cells - immunology; Dendritic Cells - metabolism; Flow Cytometry; Humans; Immune System - cytology; Immune System - immunology; Immune System - metabolism; Immunologic Memory - immunology; Immunophenotyping - methods; Inflammation - blood; Inflammation - cerebrospinal fluid; Inflammation - immunology; Killer Cells, Natural - immunology; Killer Cells, Natural - metabolism; Leukocytes - immunology; Leukocytes - metabolism; Middle Aged; Multiple Sclerosis, Chronic Progressive - blood; Multiple Sclerosis, Chronic Progressive - cerebrospinal fluid; Multiple Sclerosis, Chronic Progressive - immunology; Nervous System Malformations - blood; Nervous System Malformations - cerebrospinal fluid; Nervous System Malformations - immunology; Prospective Studies; T-Lymphocytes - immunology; T-Lymphocytes - metabolism; Young Adult

We performed unbiased, comprehensive immunophenotyping of cerebrospinal fluid (CSF) and blood leukocytes in 221 subjects referred for the diagnostic work-up of neuroimmunological disorders to obtain insight about disease-specific phenotypes of intrathecal immune responses. Quantification of 14 diffe...