Systemic lupus erythematosus: Molecular cloning of fourteen recombinant DNase monoclonal kappa light chains with different catalytic properties

• Published in: Biochimica et biophysica acta Vol. 1840; no. 6; pp. 1725 - 1737
• Main Authors: Kostrikina, Irina A., Buneva, Valentina N., Nevinsky, Georgy A.
• Format: Journal Article
• Language: English
• Published: Netherlands 01.06.2014
• Subjects: affinity chromatography; antibodies; Antibody Affinity; bacteriophages; calcium; Catalysis; cations; clones; Cloning, Molecular; cobalt; copper; deoxyribonucleases; Deoxyribonucleases - immunology; divalent metals; DNA; Escherichia coli; gel chromatography; Humans; Hydrogen-Ion Concentration; hydrolysis; Immunoglobulin kappa-Chains - genetics; immunoglobulin light chains; lupus erythematosus; Lupus Erythematosus, Systemic - immunology; lymphocytes; magnesium; Magnesium - pharmacology; manganese; Manganese - pharmacology; molecular cloning; nickel; pathogenesis; patients; potassium chloride; Recombinant Proteins - biosynthesis; sodium chloride; zinc; DNase activity; Systemic lupus erythematosus; Recombinant monoclonal light chain; Extreme diversity

DNase antibodies can play an important role in the pathogenesis of different autoimmune pathologies. An immunoglobulin light chain phagemid library derived from peripheral blood lymphocytes of patients with systemic lupus erythematosus (SLE) was used. The small pools of phage particles displaying DN...