MiR-143 and rat embryo implantation

• Published in: Biochimica et biophysica acta Vol. 1850; no. 4; pp. 708 - 721
• Main Authors: Tian, Shi, Su, Xing, Qi, Lu, Jin, Xiao-Hua, Hu, Yi, Wang, Chun-Ling, Ma, Xu, Xia, Hong-Fei
• Format: Journal Article
• Language: English
• Published: Netherlands 01.04.2015
• Subjects: Animals; bioinformatics; Cell Movement; Cell Proliferation; Embryo Implantation; endometrium; epithelium; Estradiol - pharmacology; Female; Humans; immunohistochemistry; leukemia inhibitory factor; luciferase; MicroRNAs - analysis; MicroRNAs - physiology; Northern blotting; Pregnancy; pseudopregnancy; Rats; Rats, Sprague-Dawley; Receptors, OSM-LIF - genetics; stromal cells; Uterus - metabolism; Western blotting; Embryo implantation; Rat; Uterus; miR-143; Lifr

To study the role of miR-143 during embryo implantation in rat. MiR-143 expression in rat early pregnancy was detected by Northern blot. The relation between miR-143 and Lifr predicted and confirmed by bioinformatics method, dual-luciferase activity assay, Western blot and immunohistochemistry. The...