Screening of immunogenic antigens from scuticociliate in Takifugu rubripes, and the development and evaluation of subunit vaccines

• Published in: Fish & shellfish immunology Vol. 163; p. 110385
• Main Authors: Wang, Xiaohang, Wang, Sen, Ni, Xinpeng, Zhao, Gengtong, Sun, Qingmeihui, Zhang, Ziyi, Pan, Meili, Li, Ruijun
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.08.2025
• Subjects: acetylcholinesterase; active sites; animal diseases; Animals; antigens; Antigens, Protozoan - immunology; aquaculture; centrifugation; Ciliophora - immunology; Ciliophora Infections - immunology; Ciliophora Infections - parasitology; Ciliophora Infections - prevention & control; Ciliophora Infections - veterinary; cytoplasm; disease prevention; domain; enrichment culture; excision; family; fish; Fish Diseases - immunology; Fish Diseases - parasitology; Fish Diseases - prevention & control; food safety; freeze-thaw cycles; GAPDH; gels; glyceraldehyde-3-phosphate dehydrogenase; glycolysis; Immunogenic antigen; industry; mass spectrometry; Mice; Mice, Inbred BALB C; Oligohymenophorea; parasites; parasitoses; peroxidase; polypeptides; Protozoan Vaccines - immunology; Scuticociliates; shellfish; stop codon; Subunit vaccine; subunit vaccines; superoxide dismutase; Takifugu; Takifugu rubripes; toxicity; vaccination; Vaccines, Subunit - immunology; virulent strains; Western blotting; Takifugu rubripes; Immunogenic antigen; Subunit vaccine; GAPDH; Scuticociliates

In recent years, scuticociliatosis has emerged as a major parasitic disease in the aquaculture of Takifugu rubripes, inflicting significant economic losses on the pufferfish farming industry. The conventional approach of using chemical drugs to prevent and control parasitic diseases poses substantia...