Direct Inhibition of in Vitro PLD Activity by 4-(2-Aminoethyl)-Benzenesulfonyl Fluoride

• Published in: Biochemical and biophysical research communications Vol. 273; no. 1; pp. 302 - 311
• Main Authors: Andrews, Brooke, Bond, Kristina, Lehman, Jason A., Horn, Jeffrey M., Dugan, Amy, Gomez-Cambronero, Julia
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 24.06.2000
• Subjects: Brassica - cytology; Brassica - drug effects; Brassica - enzymology; Cells, Cultured; Enzyme Inhibitors - chemistry; Enzyme Inhibitors - isolation & purification; Enzyme Inhibitors - pharmacology; Humans; Hydrogen-Ion Concentration; Inhibitory Concentration 50; neutrophils; Neutrophils - cytology; Neutrophils - drug effects; Neutrophils - enzymology; Phosphatidylcholines - metabolism; Phospholipase D - antagonists & inhibitors; Phospholipase D - isolation & purification; Phospholipase D - metabolism; Phospholipases A - metabolism; PLD; Precipitin Tests; protease inhibitor; signal transduction; Signal Transduction - drug effects; Substrate Specificity; Sulfones - chemistry; Sulfones - isolation & purification; Sulfones - pharmacology; Time Factors; Type C Phospholipases - metabolism; PLD; protease inhibitor; signal transduction; neutrophils

While conducting a purification protocol of phospholipase D (PLD) from human granulocytes, we observed that PLD activity was inhibited by a commonly-used protease inhibitor cocktail. Of the six inhibitors present in the cocktail, the serine protease inhibitor, 4-(2-aminoethyl)-benezensulfonyl fluori...