Vaccarin treats lactation insufficiency through the ALKBH5-SFRP2-Wnt/β-catenin signaling pathway

• Published in: Journal of ethnopharmacology Vol. 348; p. 119898
• Main Authors: Xue, Qiuyun, Li, Hui, Liu, Guosheng, Xiong, Youyi, Zhou, Guoliang, Xu, Pengfei, He, Juan, Wang, Xiaomei, Miao, Chenggui
• Format: Journal Article
• Language: English
• Published: Ireland Elsevier B.V 28.05.2025
• Subjects: AlkB Homolog 5, RNA Demethylase - genetics; AlkB Homolog 5, RNA Demethylase - metabolism; alkylation; Alkylation repair homolog protein 5; Animals; beta Catenin - metabolism; Cells, Cultured; childbirth; Epithelial Cells - drug effects; Epithelial Cells - metabolism; epithelium; Female; flavonoids; Flavonoids - pharmacology; Flavonoids - therapeutic use; fluorescent antibody technique; glycosides; Glycosides - pharmacology; Glycosides - therapeutic use; Gypsophila; lactation; Lactation - drug effects; Lactation Disorders - drug therapy; Lactation Disorders - metabolism; Lactation insufficiency; mammary glands; Mammary Glands, Animal - drug effects; Mammary Glands, Animal - metabolism; Membrane Proteins - genetics; Membrane Proteins - metabolism; Mice; milk production; milk secretion; precipitin tests; prolactin; prolactin receptors; Rats; Rats, Sprague-Dawley; RNA; SFRP2; traditional medicine; Vaccaria; Vaccarin; Western blotting; Wnt Signaling Pathway - drug effects; Wnt/β-catenin signaling pathway; Wnt/β-catenin signaling pathway; MT; Alkylation repair homolog protein 5; mRNA; PRL; WB; BMECs; MECs; PRLR; CK18; ALKBH5; Vaccarin; SFRP2; CCND1; RIP; Vac; RMECs; Lactation insufficiency

Vaccarin, a natural small molecule extracted from Gypsophila vaccaria (L.) Sm., is an active flavonoid glycoside. Lactation insufficiency refers to insufficient milk secretion in women after childbirth, which affects the feeding of infants and even their development. Our preliminary experiments show...