Fibroblast growth factor receptor-3 as a marker for precartilaginous stem cells
The epiphyseal organ contains two kinds of cartilage, articular and growth plate. Both enlarge during the growth phase of life. However, mitosis is not apparent in these tissues. In the current study, a search to trace the reservoirs of stem cells needed for the growth of these cartilages is done. A...
Saved in:
Published in | Clinical orthopaedics and related research no. 367 Suppl; p. S163 |
---|---|
Main Authors | , , , , , |
Format | Journal Article |
Language | English |
Published |
United States
01.10.1999
|
Subjects | |
Online Access | Get more information |
Cover
Loading…
Abstract | The epiphyseal organ contains two kinds of cartilage, articular and growth plate. Both enlarge during the growth phase of life. However, mitosis is not apparent in these tissues. In the current study, a search to trace the reservoirs of stem cells needed for the growth of these cartilages is done. A disorder in which the stem cells responsible for bone growth are mutated is achondroplasia; the mutation resides in the fibroblast growth factor receptor-3. Epiphyses stained with antifibroblast growth factor 3 antibodies reveal clusters of positively stained cells residing in the perichondrial mesenchyme, known as the ring of La Croix. Removal of the ring of La Croix causes a drastic growth arrest in the limbs of rat neonates. Cell cultures derived of the ring of La Croix biopsy specimens show high rates of cell proliferation and cell migration in vitro, in contrast to articular or growth plate derived chondrocytes. These cells stain intensely by antifibroblast growth factor receptor-3 antibodies and antiproliferative cells nuclear antigen, in contrast with articular and epiphyseal chondrocytes. Transfection of cells from the ring La Croix by an adenovirus vector containing the gene encoding for Escherichia coli beta-galactosidase (lacZ), allows tracing of these cells in tissues. Local injections were performed either to the ring of La Croix or to the joint cavity in a guinea pig model. A characteristic distribution was seen after injection. The transfected cells migrated to areas of bone and cartilage formation in the subchondral bone plate and on either side of the growth plate. This labeling and distribution is maintained for as many as 3 months after injection. The cells from the ring of La Croix appear to be responsible for bone growth. Furthermore, perichondrial cells and other precartilaginous cells expressing fibroblast growth factor-3 have been shown to be good cells for implantation to correct defects of articular cartilage. |
---|---|
AbstractList | The epiphyseal organ contains two kinds of cartilage, articular and growth plate. Both enlarge during the growth phase of life. However, mitosis is not apparent in these tissues. In the current study, a search to trace the reservoirs of stem cells needed for the growth of these cartilages is done. A disorder in which the stem cells responsible for bone growth are mutated is achondroplasia; the mutation resides in the fibroblast growth factor receptor-3. Epiphyses stained with antifibroblast growth factor 3 antibodies reveal clusters of positively stained cells residing in the perichondrial mesenchyme, known as the ring of La Croix. Removal of the ring of La Croix causes a drastic growth arrest in the limbs of rat neonates. Cell cultures derived of the ring of La Croix biopsy specimens show high rates of cell proliferation and cell migration in vitro, in contrast to articular or growth plate derived chondrocytes. These cells stain intensely by antifibroblast growth factor receptor-3 antibodies and antiproliferative cells nuclear antigen, in contrast with articular and epiphyseal chondrocytes. Transfection of cells from the ring La Croix by an adenovirus vector containing the gene encoding for Escherichia coli beta-galactosidase (lacZ), allows tracing of these cells in tissues. Local injections were performed either to the ring of La Croix or to the joint cavity in a guinea pig model. A characteristic distribution was seen after injection. The transfected cells migrated to areas of bone and cartilage formation in the subchondral bone plate and on either side of the growth plate. This labeling and distribution is maintained for as many as 3 months after injection. The cells from the ring of La Croix appear to be responsible for bone growth. Furthermore, perichondrial cells and other precartilaginous cells expressing fibroblast growth factor-3 have been shown to be good cells for implantation to correct defects of articular cartilage. |
Author | Robinson, D Cohen, N Yayon, A Hasharoni, A Moskowitz, R M Nevo, Z |
Author_xml | – sequence: 1 givenname: D surname: Robinson fullname: Robinson, D organization: Department of Clinical Biochemistry, Sackler School of Medicine, Tel Aviv University, Ramat-Aviv, Israel – sequence: 2 givenname: A surname: Hasharoni fullname: Hasharoni, A – sequence: 3 givenname: N surname: Cohen fullname: Cohen, N – sequence: 4 givenname: A surname: Yayon fullname: Yayon, A – sequence: 5 givenname: R M surname: Moskowitz fullname: Moskowitz, R M – sequence: 6 givenname: Z surname: Nevo fullname: Nevo, Z |
BackLink | https://www.ncbi.nlm.nih.gov/pubmed/10546645$$D View this record in MEDLINE/PubMed |
BookMark | eNo1T9tKAzEUzEPFXvQXJD8QzT2bRylWhUJfFHwrSfakru52lyRF_HtT1PMwcw4zDHOWaHYcj4AQZvSWUWvuaB1BG02YtZbVg5EzNDO0qGyJ5extjpY5f5yNUvFLNGdUSa2lWqDdpvNp9L3LBR_S-FXecXShjAknCDDVhQjsMnZ4cOkTEo5VmqrmUul6d-iO4ynjXGDAAfo-X6GL6PoM13-8Qq-bh5f1E9nuHp_X91sShKSFaCpEhJYG3TrgIQJTWnrFlHRaGa8arWPwNEZoogdp2mo0RnAaTRO00nyFbn5zp5MfoN1PqasFv_f_n_EfcQJSpA |
CitedBy_id | crossref_primary_10_1016_j_cden_2022_10_001 crossref_primary_10_1016_j_archoralbio_2003_12_009 crossref_primary_10_1016_j_ygeno_2021_08_015 crossref_primary_10_1002_jcp_30682 crossref_primary_10_1089_ten_tea_2010_0277 crossref_primary_10_1111_iep_12275 crossref_primary_10_1515_jpem_2010_176 crossref_primary_10_1007_s10856_015_5631_z crossref_primary_10_1007_s11596_009_0219_9 crossref_primary_10_1007_s11595_011_0282_5 crossref_primary_10_1111_j_1469_7580_2009_01115_x crossref_primary_10_3727_000000001783986882 crossref_primary_10_1016_j_bone_2003_10_003 crossref_primary_10_1007_s11596_009_0108_2 crossref_primary_10_1016_j_aanat_2016_07_003 crossref_primary_10_1074_jbc_M303164200 crossref_primary_10_1007_s11596_006_0626_0 crossref_primary_10_3390_ijms150712665 crossref_primary_10_1002_jcb_24606 crossref_primary_10_1007_s00264_006_0082_2 crossref_primary_10_1186_s12861_014_0036_7 crossref_primary_10_1007_s00418_003_0519_2 crossref_primary_10_1016_j_joca_2014_11_013 crossref_primary_10_1016_S0891_8422_23_01166_7 crossref_primary_10_1038_sj_bjc_6605407 crossref_primary_10_1038_s41392_020_00222_7 crossref_primary_10_1016_j_bbrc_2014_06_030 crossref_primary_10_1101_gad_266551_115 crossref_primary_10_1111_j_1365_2613_2009_00655_x crossref_primary_10_1002_jbm_a_34647 crossref_primary_10_1038_nature12396 crossref_primary_10_1007_s00402_008_0571_4 crossref_primary_10_1007_s11596_009_0305_z crossref_primary_10_1016_j_heliyon_2024_e32617 crossref_primary_10_1002_dvdy_24479 crossref_primary_10_3389_fbioe_2022_872149 crossref_primary_10_1016_j_joca_2021_11_017 crossref_primary_10_1007_s11596_011_0484_2 |
ContentType | Journal Article |
DBID | CGR CUY CVF ECM EIF NPM |
DOI | 10.1097/00003086-199910001-00018 |
DatabaseName | Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed |
DatabaseTitle | MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) |
DatabaseTitleList | MEDLINE |
Database_xml | – sequence: 1 dbid: NPM name: PubMed url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 2 dbid: EIF name: MEDLINE url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search sourceTypes: Index Database |
DeliveryMethod | no_fulltext_linktorsrc |
ExternalDocumentID | 10546645 |
Genre | Research Support, U.S. Gov't, Non-P.H.S Research Support, Non-U.S. Gov't Journal Article |
GroupedDBID | --- -5E -5G -BR -Y2 -~C -~X .55 .86 .GJ .VR 06C 06D 08G 0R~ 0VY 199 1CY 1KJ 1N0 203 29B 29~ 2J2 2JY 2KG 2KM 2LR 2WC 30V 354 3O- 3V. 4.4 408 40D 40E 40H 4Q1 4Q2 4Q3 53G 5GY 5RE 5VS 67Z 6J9 6NX 6PF 78A 7O~ 7RV 7X7 88E 8AO 8F7 8FI 8FJ 8TC 8UJ 95- 95. 95~ 96X AAAAV AABHQ AAEJM AAGIX AAHPQ AAIAL AAIQE AAJJC AAJKR AAMOA AANXM AAQKA AAQQT AARTL AASCR AASXQ AAWCG AAWTL AAYIU AAYQN AAYTO ABASU ABDIG ABHLI ABJNI ABJOX ABMNI ABNWP ABPLI ABPPZ ABTEG ABTMW ABUWG ABUWZ ABVCZ ACBXY ACGFO ACGFS ACGUR ACHVE ACHXU ACIHN ACILI ACKNC ACNWC ACOAL ACPRK ACREN ACRZS ACXJB ADBBV ADBIZ ADFPA ADGGA ADHIR ADHPY ADIYS ADKPE ADNKB ADQRH AE3 AEAQA AEBTG AEETU AEGNC AEJHL AEKMD AENEX AEOHA AEPYU AETLH AFBBN AFDTB AFFNX AFJLC AFKRA AFLOW AFUWQ AFWTZ AFZKB AGJBK AGQMX AGWIL AGWZB AGYKE AHAVH AHMBA AHOMT AHQNM AHRYX AHSBF AHVBC AHYZX AI. AIIXL AINUH AJBLW AJIOK AJJEV AJNWD AJRNO AJZMW AKMHD AKULP ALIPV ALMA_UNASSIGNED_HOLDINGS ALMTX ALWAN AMJPA AMKLP AMKUR AMNEI AOHHW AOIJS ARMRJ ASPBG AVWKF AWKKM AZFZN B-. BA0 BAWUL BENPR BGNMA BKEYQ BPHCQ BS7 BVXVI BYPQX C45 CAG CCPQU CGR COF CS3 CSCUP CUY CVF DIK DIWNM DUNZO E3Z EBS ECM EEVPB EIF EJD ERAAH ESBYG EX3 F5P FCALG FEDTE FIGPU FNLPD FRRFC FWDCC FYUFA G-Y G-Z GGCAI GGRSB GNXGY GQ6 GQ7 GQDEL GX1 H0~ H13 HF~ HG5 HG6 HLICF HLJTE HMCUK HMJXF HRMNR HVGLF HYE HZ~ H~9 IKREB IKYAY ITM IXC IZQ I~X I~Z J-C J0Z J5H JBSCW JF9 JG8 JK3 JK8 K8S KMI KOV KPH L7B M18 M1P M4Y MA- N9A NAPCQ NPM NU0 N~M O9- O93 O9I O9J OAG OAH OB4 OCUKA ODA OK1 OL1 OLB OLG OLH OLU OLV OLY OLZ OPUJH ORVUJ OUVQU OVD OVDNE OVIDH OWU OWV OWW OWX OWY OWZ OXXIT P-K P2P P9S PF0 PONUX PQQKQ PROAC PSQYO Q2X QOR QOS R58 R89 R9I RIG RLZ ROL RPM RPX RRX RSV S16 S1Z S27 S37 S3B S4R SAP SDH SHX SMD SNE SNX SOJ SZ9 SZN T13 T8P TEORI TR2 TSG TSK TSPGW TT1 TUC TWZ U2A U9L UG4 UKHRP VC2 VH1 VVN W48 WAF WH7 WK8 WOQ WOW X3V X3W X7M XXN XYM YCJ YFH YOC YQY YRY Z45 Z7U Z82 Z87 ZB8 ZCG ZFV ZGI ZOVNA ZXP ZZMQN |
ID | FETCH-LOGICAL-c340t-6033fed0c6dae2cfe1564b5154a657b5866fcb0ffe8fbe47dc6d77320f78c6562 |
ISSN | 0009-921X |
IngestDate | Sat Sep 28 08:37:52 EDT 2024 |
IsPeerReviewed | true |
IsScholarly | true |
Issue | 367 Suppl |
Language | English |
LinkModel | OpenURL |
MergedId | FETCHMERGED-LOGICAL-c340t-6033fed0c6dae2cfe1564b5154a657b5866fcb0ffe8fbe47dc6d77320f78c6562 |
PMID | 10546645 |
ParticipantIDs | pubmed_primary_10546645 |
PublicationCentury | 1900 |
PublicationDate | 1999-10-01 |
PublicationDateYYYYMMDD | 1999-10-01 |
PublicationDate_xml | – month: 10 year: 1999 text: 1999-10-01 day: 01 |
PublicationDecade | 1990 |
PublicationPlace | United States |
PublicationPlace_xml | – name: United States |
PublicationTitle | Clinical orthopaedics and related research |
PublicationTitleAlternate | Clin Orthop Relat Res |
PublicationYear | 1999 |
SSID | ssj0003452 |
Score | 1.884529 |
Snippet | The epiphyseal organ contains two kinds of cartilage, articular and growth plate. Both enlarge during the growth phase of life. However, mitosis is not... |
SourceID | pubmed |
SourceType | Index Database |
StartPage | S163 |
SubjectTerms | Animals Animals, Newborn Cell Division Cell Movement Cells, Cultured Chickens Chondrocytes - chemistry Epiphyses - cytology Guinea Pigs Protein-Tyrosine Kinases Rabbits Rats Receptor, Fibroblast Growth Factor, Type 3 Receptors, Fibroblast Growth Factor - analysis Stem Cell Transplantation Stem Cells - chemistry Transfection |
Title | Fibroblast growth factor receptor-3 as a marker for precartilaginous stem cells |
URI | https://www.ncbi.nlm.nih.gov/pubmed/10546645 |
hasFullText | |
inHoldings | 1 |
isFullTextHit | |
isPrint | |
link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1LT9wwELYWuPSCigoUCsiH3iKjbBzbybHiIYRUegAkOCE_ywGWFRsJwa_vjJ1s0i2opZckiiMryXyZzIxnviHkq9GuMoULTHhfs1JYwUztJCuUtUp74YRBR_H7mTy5LE-vxNVo9DSsLmnMvn15ta7kf6QK50CuWCX7DsnOJ4UTcAzyhS1IGLb_JONj8HUfDNi_TfYT3Onmtm2fk4Ea81M4YBz7yOjsHpNwHmNK4RTGcKo7bE-E-a_I5Jxh_H42NFQPuopJXNcBxxrXcxKdc6x-8bgfBMIWKskOe8U2u9VIv_tb2HReEzJfB7rWz21z6z4MEakLuoS2XrXWrC5i_5sONFyqLDYnHWjI83FSaH-o7kQJnCcGHYkJMDWuPYyx7j2p6IFEp_dRpGAcIj2--PvoAql2N7REllSFivEMgzztD5yXouga8OETtQlgHdfna7eH3LPtlAv-SbRTLj6S1dbBoN8SWtbIyE8-kR89UmhCCk1IoT1SqJ5RTRNSKCCFLiKFIlJoRMo6uTw-ujg4YW0rDWZ5mTdM5pwH73IrnfaFDR45ggzYsqWWQhlRSRmsyUPwVTC-VA4uVIoXeVCVBZO_2CDLk4eJ_0wofM91NebSWC5KU-k65K5yxsjSgTVb5FtkMz3_zTTxpdx0b2b7zZEv5EOPqR2yEuAD9btg7TVmL8rmF-zRUWc |
link.rule.ids | 786 |
linkProvider | National Library of Medicine |
openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Fibroblast+growth+factor+receptor-3+as+a+marker+for+precartilaginous+stem+cells&rft.jtitle=Clinical+orthopaedics+and+related+research&rft.au=Robinson%2C+D&rft.au=Hasharoni%2C+A&rft.au=Cohen%2C+N&rft.au=Yayon%2C+A&rft.date=1999-10-01&rft.issn=0009-921X&rft.issue=367+Suppl&rft.spage=S163&rft_id=info:doi/10.1097%2F00003086-199910001-00018&rft_id=info%3Apmid%2F10546645&rft_id=info%3Apmid%2F10546645&rft.externalDocID=10546645 |
thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=0009-921X&client=summon |
thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=0009-921X&client=summon |
thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=0009-921X&client=summon |