Measuring Mitochondrial Substrate Utilization in Skeletal Muscle Stem Cells

• Published in: Methods in molecular biology (Clifton, N.J.) Vol. 1668; p. 61
• Main Authors: Ly, C Hai, Ryall, James G
• Format: Journal Article
• Language: English
• Published: United States 2017
• Subjects: Animals; Cell Differentiation - drug effects; Cell Line; Cell Proliferation - drug effects; Epoxy Compounds - pharmacology; Fatty Acids - metabolism; Glucose - metabolism; Glutamic Acid - metabolism; Hydrogen-Ion Concentration; Hypoglycemic Agents - pharmacology; Mitochondria, Muscle - drug effects; Mitochondria, Muscle - metabolism; Myoblasts, Skeletal - drug effects; Myoblasts, Skeletal - metabolism; Oxygen Consumption - drug effects; Smegmamorpha; Sulfides - pharmacology; Thiadiazoles - pharmacology; Fatty acid oxidation; Glycolysis; Glutaminolysis; Metabolism; Muscle stem cells

Skeletal muscle stem cells (MuSCs) derived from the somatic mesoderm play a critical role in successful muscle regeneration following injury and trauma. MuSCs have been found to undergo rapid changes in metabolism following a change in cell state, such as that which occurs during the transition from...