Clonal selection of high producing, stably transfected HEK293 cell lines utilizing modified, high-throughput FACS screening

• Published in: Journal of chemical technology and biotechnology (1986) Vol. 86; no. 7; pp. 935 - 941
• Main Authors: Song, Michael, Raphaelli, Kristin, Jones, Martina L., Aliabadi-Zadeh, Khosrow, Leung, Kar Man, Crowley, David, Hughes, Benjamin, Mahler, Stephen, Gray, Peter P., Huang, Edwin P., Chin, David Y.
• Format: Journal Article
• Language: English
• Published: Chichester, UK John Wiley & Sons, Ltd 01.07.2011
• Subjects: cold capture; FACS; HEK-293; monoclonal antibody; stable transfection

BACKGROUND: Human embryonic kidney‐293 (HEK‐293) cells are commonly used as a transient expression host but their application in stable therapeutic protein production is limited. This is presumably due to the absence of a suitable amplifiable expression system and hence limited protein output compar...