Study of characterization and application on the binding between 5-iodouridine with HSA by spectroscopic and modeling

• Published in: Carbohydrate polymers Vol. 73; no. 3; pp. 464 - 472
• Main Authors: Cui, Fengling, Zhang, Qiangzhai, Yan, Yinghua, Yao, Xiaojun, Qu, Guirong, Lu, Yan
• Format: Journal Article
• Language: English
• Published: Oxford Elsevier Ltd 01.08.2008; Elsevier Science
• Subjects: 5-Iodouridine; Applied sciences; Biological and medical sciences; Determination; Exact sciences and technology; Fluorescence quenching; Fundamental and applied biological sciences. Psychology; Human serum albumin (HSA); In solution. Condensed state. Thin layers; Modeling; Molecular biophysics; Natural polymers; Physico-chemical properties of biomolecules; Physicochemistry of polymers; Proteins; Synchronous fluorescence; 5-Iodouridine; Determination; Synchronous fluorescence; Human serum albumin (HSA); Modeling; Fluorescence quenching; Serum albumin; Experimental study; Iodine Organic compounds; Protein; Intermolecular interaction; Biological fixation; Salt effect; Molecular model; Binding mode; Nucleoside; Aqueous solution

The binding of 5-iodouridine with human serum albumin was investigated under the simulative physiological conditions. The fluorescence spectra in combination with UV absorption and modeling method were used in the present work. A strong fluorescence quenching reaction of 5-iodouridine to HSA was obs...