Quantifying catecholamines using multi-way kinetic modelling

• Published in: Analytica chimica acta Vol. 475; no. 1; pp. 137 - 150
• Main Authors: Nikolajsen, Rikke P.H, Booksh, Karl S, Hansen, Åse M, Bro, Rasmus
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 03.01.2003; Elsevier
• Subjects: Analysis of complex biological substances; Analytical, structural and metabolic biochemistry; Biological and medical sciences; Blood and biological fluids; Catecholamines; Fundamental and applied biological sciences. Psychology; Kinetics; PARAFAC; Stress; Trihydroxyindole; Urine; Urine; Catecholamines; Kinetics; Trihydroxyindole; Stress; PARAFAC; Epinephrine; Biological fluid; Parallel algorithm; Data analysis; Biochemical analysis; Factor analysis; Catecholamine; Fluorescence spectrometry; PLS regression; Multicomponent analysis; Analytical reagent; Norepinephrine; Indole derivatives; Chemometrics; Quantitative analysis

A new method for quantifying adrenaline and noradrenaline concentrations from mixtures of catecholamine standards is described. The method derives selectivity from the different rates, at which the fluorescing 3,5,6-trihydroxyindole derivatives (lutines) of the catecholamines are formed and degraded...