Autocrine Inhibition of the Epidermal Growth Factor Receptor by Intracellular Expression of a Single-Chain Antibody

A gene encoding a single-chain antibody which specifically binds the human epidermal growth factor (EGF) receptor has been constructed and expressed intracellularly. The single-chain antibody is derived from monoclonal antibody 225 which competes with EGF for binding to the extracellular domain of t...

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Bibliographic Details
Published inBiochemical and biophysical research communications Vol. 204; no. 2; pp. 666 - 672
Main Authors Beerli, R.R., Wels, W., Hynes, N.E.
Format Journal Article
LanguageEnglish
Published United States Elsevier Inc 28.10.1994
Subjects
3T3 Cells
Amino Acid Sequence
Animals
Cell Division - immunology
Cell Line, Transformed
Humans
Immunoglobulin Fragments - immunology
Mice
Molecular Sequence Data
Receptor, Epidermal Growth Factor - antagonists & inhibitors
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Summary:A gene encoding a single-chain antibody which specifically binds the human epidermal growth factor (EGF) receptor has been constructed and expressed intracellularly. The single-chain antibody is derived from monoclonal antibody 225 which competes with EGF for binding to the extracellular domain of the receptor. The single-chain antibody was provided with a signal peptide to direct it to the secretory pathway and was expressed in EGF receptor transformed NIH/3T3 fibroblasts. EGF induced activation of its receptor was reduced in these cells. In addition, EGF-induced anchorage-independent growth of the cells was inhibited. The data suggest that the single-chain antibody functions in an autocrine fashion to inhibit the activity of the EGF receptor.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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ISSN:0006-291X
1090-2104
DOI:10.1006/bbrc.1994.2511