Inhibition of G1 Cyclin Expression and G1 Cyclin-Dependent Protein-Kinases by cAMP in an Astrocytic Cell Line

• Published in: Biochemical and biophysical research communications Vol. 205; no. 1; pp. 923 - 929
• Main Authors: Gagelin, C., Pierre, M., Torudelbauffe, D.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 30.11.1994
• Subjects: Astrocytes - cytology; CDC2-CDC28 Kinases; Cell Division; Cell Line; Cyclic AMP - physiology; Cyclin-Dependent Kinase 2; Cyclin-Dependent Kinases - antagonists & inhibitors; Cyclin-Dependent Kinases - metabolism; Cyclins - antagonists & inhibitors; Cyclins - metabolism; Enzyme Activation; Immunohistochemistry; Kinetics; Mitogen-Activated Protein Kinase 1; Protein-Serine-Threonine Kinases - antagonists & inhibitors; Protein-Serine-Threonine Kinases - metabolism; Protein-Tyrosine Kinases - metabolism

The effects of cAMP on cell cycle progression were examined using an astrocytic cell line. We show that forskolin and 8-bromo-cAMP block the basic Fibroblast Growth Factor-induced DNA synthesis, do not inhibit mitogen activated protein kinase activation whereas they reduce G1 cyclin (E and D1) expre...