Inhibition of G1 Cyclin Expression and G1 Cyclin-Dependent Protein-Kinases by cAMP in an Astrocytic Cell Line

The effects of cAMP on cell cycle progression were examined using an astrocytic cell line. We show that forskolin and 8-bromo-cAMP block the basic Fibroblast Growth Factor-induced DNA synthesis, do not inhibit mitogen activated protein kinase activation whereas they reduce G1 cyclin (E and D1) expre...

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Bibliographic Details
Published inBiochemical and biophysical research communications Vol. 205; no. 1; pp. 923 - 929
Main Authors Gagelin, C., Pierre, M., Torudelbauffe, D.
Format Journal Article
LanguageEnglish
Published United States Elsevier Inc 30.11.1994
Subjects
Astrocytes - cytology
CDC2-CDC28 Kinases
Cell Division
Cell Line
Cyclic AMP - physiology
Cyclin-Dependent Kinase 2
Cyclin-Dependent Kinases - antagonists & inhibitors
Cyclin-Dependent Kinases - metabolism
Cyclins - antagonists & inhibitors
Cyclins - metabolism
Enzyme Activation
Immunohistochemistry
Kinetics
Mitogen-Activated Protein Kinase 1
Protein-Serine-Threonine Kinases - antagonists & inhibitors
Protein-Serine-Threonine Kinases - metabolism
Protein-Tyrosine Kinases - metabolism
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Summary:The effects of cAMP on cell cycle progression were examined using an astrocytic cell line. We show that forskolin and 8-bromo-cAMP block the basic Fibroblast Growth Factor-induced DNA synthesis, do not inhibit mitogen activated protein kinase activation whereas they reduce G1 cyclin (E and D1) expression without modification of cyclin A level, Furthermore, they inhibit the activation of cyclin A- and cyclin E-dependent histone H1 kinases. These results suggest that cAMP may exert its antiproliferative effects through the regulation of cyclin synthesis and cyclin-dependent kinase activation.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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ISSN:0006-291X
1090-2104
DOI:10.1006/bbrc.1994.2753