Analysis of a Human cDNA Containing a Tissue-Specific Alternatively Spliced LIM Domain

• Published in: Biochemical and biophysical research communications Vol. 252; no. 2; pp. 433 - 439
• Main Authors: Putilina, T., Jaworski, C., Gentleman, S., McDonald, B., Kadiri, M., Wong, P.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 18.11.1998
• Subjects: Adult; Alternative Splicing; Amino Acid Sequence; Animals; Base Sequence; Cloning, Molecular; Consensus Sequence; DNA, Complementary - genetics; Fetus - metabolism; Genetic Variation; Homeodomain Proteins - genetics; Humans; LIM Domain Proteins; Molecular Sequence Data; Nerve Tissue - metabolism; Pancreas - metabolism; Reverse Transcriptase Polymerase Chain Reaction; Sequence Homology, Amino Acid; Tissue Distribution; Transcription Factors - genetics
• ISSN: 0006-291X; 1090-2104
• DOI: 10.1006/bbrc.1998.9656

A unique clone, isolated from a human pancreatic cDNA library, was sequenced and characterized. Northern blot analysis showed that the gene is active in a number of fetal and adult tissues, and immunoblots showed expression in nuclear and cytosolic cell fractions. The gene corresponding to the clone was localized to chromosome 13 by human/rodent somatic cell hybrid panels. The largest open reading frame contains a LIM domain, and the deduced peptide from the open reading frame appears to have the characteristics of a LIM-only protein, designatedLMO7.RT-PCR and genomic sequence analyses indicate that expression of this gene product is subject to tissue-specific modulation by elimination of the LIM domain by alternative splicing in neural tissues.