Transcription of Hepatic Cytosolic Phosphoenolpyruvate Carboxykinase Gene in Newborn Dogs

• Published in: Biochemical and molecular medicine Vol. 59; no. 1; pp. 13 - 19
• Main Authors: Feng, Bing-cheng, Li, Jixuan, Kliegman, Robert M.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.10.1996
• Subjects: Animals; Animals, Newborn - genetics; Animals, Newborn - metabolism; Blood Glucose - metabolism; Blotting, Northern; Cytosol - enzymology; Dogs; Epinephrine - blood; Fasting; Insulin - blood; Liver - enzymology; Phosphoenolpyruvate Carboxykinase (GTP) - genetics; Phosphoenolpyruvate Carboxykinase (GTP) - metabolism; RNA, Messenger - metabolism; Transcription, Genetic

Physiological studies hypothesized that unsuppressed gluconeogenesis by insulin in newborn dogs may be a mechanism responsible for neonatal hyperglycemia. In the present study, we determined the effects of fasting and the infusion of insulin, glucose, and/or epinephrine on the liver cytosolic mRNA l...