The timing of retroviral silencing correlates with the quality of induced pluripotent stem cell lines

• Published in: Biochimica et biophysica acta Vol. 1810; no. 2; pp. 226 - 235
• Main Authors: Okada, Minoru, Yoneda, Yoshihiro
• Format: Journal Article
• Language: English
• Published: Netherlands 01.02.2011
• Subjects: adults; Animals; Cell Line; Cells, Cultured; chimerism; clones; Embryo, Mammalian - cytology; Embryonic Stem Cells - cytology; Embryonic Stem Cells - metabolism; Female; fibroblasts; Fibroblasts - cytology; Fibroblasts - metabolism; Gene Expression Profiling; germ cells; green fluorescent protein; Green Fluorescent Proteins - genetics; Green Fluorescent Proteins - metabolism; Homeodomain Proteins - genetics; Homeodomain Proteins - metabolism; Induced Pluripotent Stem Cells - cytology; Induced Pluripotent Stem Cells - metabolism; Karyotyping; Kruppel-Like Transcription Factors - genetics; Kruppel-Like Transcription Factors - metabolism; Male; Mice; Mice, Inbred C57BL; Mice, Inbred ICR; Microscopy, Fluorescence; Nanog Homeobox Protein; Octamer Transcription Factor-3 - genetics; Octamer Transcription Factor-3 - metabolism; Proto-Oncogene Proteins c-myc - genetics; Proto-Oncogene Proteins c-myc - metabolism; Retroviridae; Retroviridae - genetics; Reverse Transcriptase Polymerase Chain Reaction; somatic cells; SOXB1 Transcription Factors - genetics; SOXB1 Transcription Factors - metabolism; stem cells; Time Factors; transcription factors; Transduction, Genetic; transgenes

Induced pluripotent stem (iPS) cells can be generated from somatic cells by introducing the four transcription factors Oct4, Sox2, Klf4, and c-Myc. Given that iPS cell technology may be useful for medical applications, the quality of iPS cells needs to be maintained during prolonged cultivation. How...