A novel 4-hydroxypyrene-based “off–on” fluorescent probe with large Stokes shift for detecting cysteine and its application in living cells

• Published in: Tetrahedron letters Vol. 61; no. 6; pp. 151467 - 151472
• Main Authors: Sun, Wenhao, Tang, Xinxue, Li, Jingyang, He, Menglu, Zhang, Ran, Han, Xiang'en, Zhao, Yun, Ni, Zhonghai
• Format: Journal Article
• Language: English
• Published: OXFORD Elsevier Ltd 06.02.2020; Elsevier
• Subjects: 4-Acrylatepyrene; Cell imaging; Chemistry; Chemistry, Organic; cyclization reactions; Cys detection; cysteine; cytotoxicity; detection limit; fluorescent dyes; glutathione; high performance liquid chromatography; homocysteine; image analysis; ions; Large Stokes shift; Lewis bases; moieties; nuclear magnetic resonance spectroscopy; Physical Sciences; Science & Technology; Large Stokes shift; Cell imaging; 4-Acrylatepyrene; Cys detection; HIGHLY SELECTIVE DETECTION; HOMOCYSTEINE; THIOLS; BODIPY; LIQUID-CHROMATOGRAPHY; HUMAN PLASMA; FILM; GLUTATHIONE

[Display omitted] •A new highly sensitive probe that capable of specifically detecting cysteine was developed.•The fluorescence of PYAC exhibits “off–on” switching property and a large Stokes shift after the addition of Cys.•The detect limitation is down to 0.062 μM.•The probe has negligible toxicit...