Protein–lipid interactions during the incubation of whey proteins with autoxidizing lipids

The changes induced in whey proteins due to incubation with oils containing different amounts of polyunsaturated fatty acids (fish, sunflower, soybean and olive oil) and with different initial oxidation status were studied under autoxidizing conditions. Changes upon incubation with fresh vegetable o...

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Published inInternational dairy journal Vol. 21; no. 6; pp. 427 - 433
Main Authors Cucu, Tatiana, Devreese, Bart, Mestdagh, Frédéric, Kerkaert, Barbara, De Meulenaer, Bruno
Format Journal Article
LanguageEnglish
Published Oxford Elsevier Ltd 01.06.2011
Elsevier
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Abstract The changes induced in whey proteins due to incubation with oils containing different amounts of polyunsaturated fatty acids (fish, sunflower, soybean and olive oil) and with different initial oxidation status were studied under autoxidizing conditions. Changes upon incubation with fresh vegetable oils were very limited. Only upon incubation with fish or highly oxidized soybean oil, most significant changes with respect to lysine, histidine, phenylalanine and arginine residues occurred. Remarkably, the content of methionine and tryptophan remained unchanged under the experimental conditions. The most prominent impact of incubation of whey proteins with lipids was severe protein aggregation as shown by sodium dodecyl sulphate-polyacrylamide gel electrophoresis.
AbstractList The changes induced in whey proteins due to incubation with oils containing different amounts of polyunsaturated fatty acids (fish, sunflower, soybean and olive oil) and with different initial oxidation status were studied under autoxidizing conditions. Changes upon incubation with fresh vegetable oils were very limited. Only upon incubation with fish or highly oxidized soybean oil, most significant changes with respect to lysine, histidine, phenylalanine and arginine residues occurred. Remarkably, the content of methionine and tryptophan remained unchanged under the experimental conditions. The most prominent impact of incubation of whey proteins with lipids was severe protein aggregation as shown by sodium dodecyl sulphate-polyacrylamide gel electrophoresis.
Author Cucu, Tatiana
De Meulenaer, Bruno
Mestdagh, Frédéric
Devreese, Bart
Kerkaert, Barbara
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  givenname: Frédéric
  surname: Mestdagh
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  organization: Department of Food Safety and Food Quality, Research Group Food Chemistry and Human Nutrition, Ghent University, Coupure Links 653, B-9000 Gent, Belgium
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  givenname: Barbara
  surname: Kerkaert
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  surname: De Meulenaer
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  organization: Department of Food Safety and Food Quality, Research Group Food Chemistry and Human Nutrition, Ghent University, Coupure Links 653, B-9000 Gent, Belgium
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Issue 6
Keywords Lipids
Milk protein
Incubation
Whey protein
Interaction
Dairy industry
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Snippet The changes induced in whey proteins due to incubation with oils containing different amounts of polyunsaturated fatty acids (fish, sunflower, soybean and...
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SubjectTerms Biological and medical sciences
Food industries
Fundamental and applied biological sciences. Psychology
Milk and cheese industries. Ice creams
Title Protein–lipid interactions during the incubation of whey proteins with autoxidizing lipids
URI https://dx.doi.org/10.1016/j.idairyj.2011.01.003
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