Design of Experiment (DOE) Utilization to Develop a Simple and Robust Reversed-Phase HPLC Technique for Related Substances' Estimation of Omeprazole Formulations

• Published in: Scientia pharmaceutica Vol. 81; no. 4; pp. 1043 - 1056
• Main Authors: Manranjan, Vayeda Chintan, Yadav, Devendra Singh, Jogia, Hitesh Amrutlal, Chauhan, Praful Lalitkumar
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 01.10.2013; Österreichische Apotheker-Verlagsgesellschaft
• Subjects: Related Substances; Method development; Anova; DOE; HPLC/UPLC; Compatible; Chromatography

A simple, fast, and sensitive reversed-phase HPLC method with UV detection was developed for the quantitation of omeprazole and its eleven related compounds (impurities) in pharmaceutical formulation using the Thermo Accucore C-18 (50 mm × 4.6 mm, 2.6 μm) column. The separation among all the compoun...