Up-Regulation of p21WAF1 by Phorbol Ester and Calcium in Human Keratinocytes through a Protein Kinase C-Dependent Pathway

• Published in: The American journal of pathology Vol. 153; no. 1; pp. 39 - 45
• Main Authors: Todd, Carole, Reynolds, Nicholas J
• Format: Journal Article
• Language: English
• Published: Bethesda, MD ASIP 01.07.1998; American Society for Investigative Pathology
• Subjects: Biological and medical sciences; Blotting, Western; Calcium - pharmacology; Cell Differentiation - drug effects; Cells, Cultured; Cyclin-Dependent Kinase Inhibitor p21; Cyclins - metabolism; Enzyme Inhibitors - metabolism; General aspects; Humans; Immunoenzyme Techniques; Keratinocytes - metabolism; Medical sciences; Protein Kinase C - physiology; Psychology. Psychoanalysis. Psychiatry; Psychopathology. Psychiatry; Short Communication; Signal Transduction; Tetradecanoylphorbol Acetate - pharmacology; Time Factors; Tumor Suppressor Protein p53 - metabolism; Up-Regulation; Human; Immunohistochemistry; Cell culture; Skin disease; Squamous cell carcinoma; Protein kinase C; Calcium; Psoriasis; Enzyme; Pathogenesis; Transferases; Keratinization; Malignant tumor; Cell differentiation; In vitro; Pathology; Regulation(control); C-Onc gene; Immunoblotting assay; Keratinocyte; Skin; Molecular biology; Protooncogene

Terminal differentiation in a variety of cell types has been associated with p53-independent up-regulation of p21WAF1 p21WAF1 mRNA and protein are expressed at low levels in normal human skin, but overexpression of p21WAF1 has been observed in differentiating keratinocytes in involved psoriatic epid...