Simultaneous detection of lead (II) and mercury (II) ions using nucleic acid aptamer molecular beacons
We have developed a fluorescence quantitative analysis method for the simultaneous detection of Hg 2+ and Pb 2+ based on nucleic acid aptamer molecular beacon (MB) probes. In this analytical method, two MB probes for Hg 2+ (P Hg ) and Pb 2+ (P Pb ) were designed. The carboxyl fluorescein (FAM) and t...
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Published in | International journal of environmental analytical chemistry Vol. 101; no. 13; pp. 1922 - 1934 |
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Main Authors | , , , , , , |
Format | Journal Article |
Language | English |
Published |
Abingdon
Taylor & Francis
21.10.2021
Taylor & Francis LLC |
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Abstract | We have developed a fluorescence quantitative analysis method for the simultaneous detection of Hg
2+
and Pb
2+
based on nucleic acid aptamer molecular beacon (MB) probes. In this analytical method, two MB probes for Hg
2+
(P
Hg
) and Pb
2+
(P
Pb
) were designed. The carboxyl fluorescein (FAM) and tetramethyl-6-carboxyrhodamine (TAMRA) were selected as fluorophores of P
Hg
and P
Pb
, Black Hole Quencher 1 (BHQ-1) and Black Hole Quencher 2 (BHQ-2) were selected as organic quenchers, and several continuous nucleotides with guanine (G base) were connected to organic quenchers. The aptamers are put in as a part of stem and loop. In general, the fluorescence of fluorophores was dually quenched by BHQ and G bases, so the fluorescence signals were weak. In the presence of Hg
2+
and Pb
2+
, P
Hg
and P
Pb
bonded with them, and the stem-loop structure of MB was destroyed and the fluorescence recovered. Under the optimal conditions, the fluorescence intensity of FAM had a good linear relationship with the concentration of Hg
2+
in the range from 0.7 nmol/L to 84 nmol/L, and that of TAMRA and Pb
2+
in the range from 0.2 nmol/L to 24 nmol/L. The detection limit of Hg
2+
is 0.36 nmol/L and that of Pb
2+
is 0.16 nmol/L (3σ, n = 11). The relative standard deviations (RSD) for determination of Hg
2+
and Pb
2+
were both lower than 5%, the average recoveries of this method in real samples were 96.55-102.78%, which indicated that the method had a high accuracy. |
---|---|
AbstractList | We have developed a fluorescence quantitative analysis method for the simultaneous detection of Hg
2+
and Pb
2+
based on nucleic acid aptamer molecular beacon (MB) probes. In this analytical method, two MB probes for Hg
2+
(P
Hg
) and Pb
2+
(P
Pb
) were designed. The carboxyl fluorescein (FAM) and tetramethyl-6-carboxyrhodamine (TAMRA) were selected as fluorophores of P
Hg
and P
Pb
, Black Hole Quencher 1 (BHQ-1) and Black Hole Quencher 2 (BHQ-2) were selected as organic quenchers, and several continuous nucleotides with guanine (G base) were connected to organic quenchers. The aptamers are put in as a part of stem and loop. In general, the fluorescence of fluorophores was dually quenched by BHQ and G bases, so the fluorescence signals were weak. In the presence of Hg
2+
and Pb
2+
, P
Hg
and P
Pb
bonded with them, and the stem-loop structure of MB was destroyed and the fluorescence recovered. Under the optimal conditions, the fluorescence intensity of FAM had a good linear relationship with the concentration of Hg
2+
in the range from 0.7 nmol/L to 84 nmol/L, and that of TAMRA and Pb
2+
in the range from 0.2 nmol/L to 24 nmol/L. The detection limit of Hg
2+
is 0.36 nmol/L and that of Pb
2+
is 0.16 nmol/L (3σ, n = 11). The relative standard deviations (RSD) for determination of Hg
2+
and Pb
2+
were both lower than 5%, the average recoveries of this method in real samples were 96.55-102.78%, which indicated that the method had a high accuracy. We have developed a fluorescence quantitative analysis method for the simultaneous detection of Hg2+ and Pb2+ based on nucleic acid aptamer molecular beacon (MB) probes. In this analytical method, two MB probes for Hg2+ (PHg) and Pb2+ (PPb) were designed. The carboxyl fluorescein (FAM) and tetramethyl-6-carboxyrhodamine (TAMRA) were selected as fluorophores of PHg and PPb, Black Hole Quencher 1 (BHQ-1) and Black Hole Quencher 2 (BHQ-2) were selected as organic quenchers, and several continuous nucleotides with guanine (G base) were connected to organic quenchers. The aptamers are put in as a part of stem and loop. In general, the fluorescence of fluorophores was dually quenched by BHQ and G bases, so the fluorescence signals were weak. In the presence of Hg2+ and Pb2+, PHg and PPb bonded with them, and the stem-loop structure of MB was destroyed and the fluorescence recovered. Under the optimal conditions, the fluorescence intensity of FAM had a good linear relationship with the concentration of Hg2+ in the range from 0.7 nmol/L to 84 nmol/L, and that of TAMRA and Pb2+ in the range from 0.2 nmol/L to 24 nmol/L. The detection limit of Hg2+ is 0.36 nmol/L and that of Pb2+ is 0.16 nmol/L (3σ, n = 11). The relative standard deviations (RSD) for determination of Hg2+ and Pb2+ were both lower than 5%, the average recoveries of this method in real samples were 96.55–102.78%, which indicated that the method had a high accuracy. |
Author | Wang, Peng Xiong, Weiwei Zhai, Kun Shi, Rujie Lu, Zijing Xiang, Dongshan Li, Xin |
Author_xml | – sequence: 1 givenname: Zijing surname: Lu fullname: Lu, Zijing organization: Hubei Minzu University – sequence: 2 givenname: Weiwei surname: Xiong fullname: Xiong, Weiwei organization: Hubei Minzu University – sequence: 3 givenname: Peng surname: Wang fullname: Wang, Peng organization: Hubei Minzu University – sequence: 4 givenname: Xin surname: Li fullname: Li, Xin organization: Hubei Minzu University – sequence: 5 givenname: Kun surname: Zhai fullname: Zhai, Kun organization: Hubei Minzu University – sequence: 6 givenname: Rujie surname: Shi fullname: Shi, Rujie email: 281926472@qq.com organization: Chongqing Three Gorges University – sequence: 7 givenname: Dongshan surname: Xiang fullname: Xiang, Dongshan email: zk3100@sohu.com organization: Hubei Minzu University |
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Cites_doi | 10.1039/C8CP01718B 10.1289/ehp.1002720 10.1093/nar/gkv1320 10.1016/j.bios.2012.07.045 10.2116/analsci.33.275 10.1016/j.talanta.2011.01.016 10.1016/j.microc.2018.03.017 10.1038/355850a0 10.1021/ac8022185 10.1016/j.microc.2017.09.007 10.1016/j.trac.2007.12.004 10.1016/j.talanta.2013.09.013 10.1016/j.talanta.2018.01.061 10.1016/j.scitotenv.2017.09.126 10.1080/19393210.2012.680612 10.1021/ja0446202 10.1016/j.sab.2016.02.017 10.1039/C6AN00879H 10.1016/j.talanta.2017.06.020 10.1002/marc.200500837 10.1016/j.aca.2012.04.020 10.1016/j.envres.2013.07.005 10.1016/j.microc.2018.06.019 10.3390/s19030599 10.1006/abio.1996.0477 10.1016/j.ccr.2012.05.012 10.1021/acs.analchem.6b02466 10.1007/s12011-017-1090-3 10.1016/j.snb.2017.11.110 |
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Snippet | We have developed a fluorescence quantitative analysis method for the simultaneous detection of Hg
2+
and Pb
2+
based on nucleic acid aptamer molecular beacon... We have developed a fluorescence quantitative analysis method for the simultaneous detection of Hg2+ and Pb2+ based on nucleic acid aptamer molecular beacon... |
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StartPage | 1922 |
SubjectTerms | Acoustic transponders aptamer Aptamers Bonding strength Chemical compounds Detection Fluorescein Fluorescence Fluorophores Guanine Ion probes Lead Lead (II) Mercury Mercury (II) Mercury (metal) Nucleic acids Nucleotides Probes Sensors simultaneous detection |
Title | Simultaneous detection of lead (II) and mercury (II) ions using nucleic acid aptamer molecular beacons |
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