IGF1 promotes human myotube differentiation toward a mature metabolic and contractile phenotype

• Published in: American Journal of Physiology: Cell Physiology Vol. 326; no. 5; pp. C1462 - C1481
• Main Authors: Dreher, Simon I, Grubba, Paul, von Toerne, Christine, Moruzzi, Alessia, Maurer, Jennifer, Goj, Thomas, Birkenfeld, Andreas L, Peter, Andreas, Loskill, Peter, Hauck, Stefanie M, Weigert, Cora
• Format: Journal Article
• Language: English
• Published: United States American Physiological Society 01.05.2024
• Subjects: CD56 antigen; Cell Differentiation; Cells, Cultured; Diabetes mellitus (insulin dependent); Diabetes mellitus (non-insulin dependent); Electron transport; Glucose; Glucose - metabolism; Glucose transporter; Glucose Transporter Type 4 - genetics; Glucose Transporter Type 4 - metabolism; Growth factors; Humans; Insulin; Insulin-like growth factor I; Insulin-Like Growth Factor I - metabolism; Muscle Contraction; Muscle Fibers, Skeletal - drug effects; Muscle Fibers, Skeletal - metabolism; Muscle, Skeletal - drug effects; Muscle, Skeletal - metabolism; Muscle, Skeletal - physiology; Musculoskeletal system; Myoblasts; Myosin Heavy Chains - genetics; Myosin Heavy Chains - metabolism; Myotubes; Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha - genetics; Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha - metabolism; Phenotype; Phenotypes; Physiology; Proteomes; Serum-free medium; Skeletal muscle; EPS; contraction; proteomics; GLUT4; human myotubes

Skeletal muscle mediates the beneficial effects of exercise, thereby improving insulin sensitivity and reducing the risk for type 2 diabetes. Current human skeletal muscle models in vitro are incapable of fully recapitulating its physiological functions especially muscle contractility. By supplement...