Effect of 4-phenylbutyrate addition timing on titer of Fc-fusion protein in Chinese hamster ovary cell cultures

Chinese hamster ovary (CHO) cells have been widely used in the biotechnology industry for the production of therapeutic proteins, leading to various research efforts to increase the productivity of therapeutic proteins. Although the addition of small molecule enhancers in CHO cell culture can enhanc...

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Bibliographic Details
Published inBiotechnology and bioprocess engineering Vol. 29; no. 4; pp. 712 - 720
Main Authors Lim, Jungae, Lim, Jin-Hyuk, Lee, Ji-Hoon, Cheon, Su-Hwan, Lee, Guewha, Kim, Z-Hun, Kim, Dong-Il
Format Journal Article
LanguageEnglish
Published Seoul The Korean Society for Biotechnology and Bioengineering 01.08.2024
Springer Nature B.V
한국생물공학회
Subjects
Acids
Antibodies
Apoptosis
Bioengineering
Biotechnology
Biotechnology industry
Cell culture
Cell cycle
Cell death
Cell fusion
Chemistry
Chemistry and Materials Science
Cricetulus griseus
Development strategies
Engineering
Fc receptors
Fusion protein
growth retardation
Industrial and Production Engineering
industry
Ovaries
Phenylbutyric acid
Product quality
Productivity
Protein folding
protein synthesis
Proteins
recombinant proteins
Research Paper
therapeutics
생물공학
4-phenylbutyric acid
Mammalian cell culture
Chinese hamster ovary cell cultures
Recombinant proteins
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Summary:Chinese hamster ovary (CHO) cells have been widely used in the biotechnology industry for the production of therapeutic proteins, leading to various research efforts to increase the productivity of therapeutic proteins. Although the addition of small molecule enhancers in CHO cell culture can enhance productivity, it poses a problem by negatively affecting product quality. In this study, effects of 4-phenylbutyrate (4-PBA), a chemical chaperone having a positive influence on recombinant protein production in CHO cells, were investigated. By varying the timing of 4-PBA addition, it was confirmed that the titer could be increased by 1.57-fold while reducing negative effects such as growth inhibition and apoptosis, thus preserving product quality. These effects of 4-PBA demonstrate the potential of using additive for developing novel enhancers for the production of recombinant proteins and the development of strategies for CHO cell engineering.
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ISSN:1226-8372
1976-3816
DOI:10.1007/s12257-024-00105-6