Effect of 4-phenylbutyrate addition timing on titer of Fc-fusion protein in Chinese hamster ovary cell cultures
Chinese hamster ovary (CHO) cells have been widely used in the biotechnology industry for the production of therapeutic proteins, leading to various research efforts to increase the productivity of therapeutic proteins. Although the addition of small molecule enhancers in CHO cell culture can enhanc...
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| Published in | Biotechnology and bioprocess engineering Vol. 29; no. 4; pp. 712 - 720 |
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| Main Authors | , , , , , , |
| Format | Journal Article |
| Language | English |
| Published |
Seoul
The Korean Society for Biotechnology and Bioengineering
01.08.2024
Springer Nature B.V 한국생물공학회 |
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| Abstract | Chinese hamster ovary (CHO) cells have been widely used in the biotechnology industry for the production of therapeutic proteins, leading to various research efforts to increase the productivity of therapeutic proteins. Although the addition of small molecule enhancers in CHO cell culture can enhance productivity, it poses a problem by negatively affecting product quality. In this study, effects of 4-phenylbutyrate (4-PBA), a chemical chaperone having a positive influence on recombinant protein production in CHO cells, were investigated. By varying the timing of 4-PBA addition, it was confirmed that the titer could be increased by 1.57-fold while reducing negative effects such as growth inhibition and apoptosis, thus preserving product quality. These effects of 4-PBA demonstrate the potential of using additive for developing novel enhancers for the production of recombinant proteins and the development of strategies for CHO cell engineering. |
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| AbstractList | Chinese hamster ovary (CHO) cells have been widely used in the biotechnology industry for the production of therapeutic proteins, leading to various research efforts to increase the productivity of therapeutic proteins. Although the addition of small molecule enhancers in CHO cell culture can enhance productivity, it poses a problem by negatively affecting product quality. In this study, effects of 4-phenylbutyrate (4-PBA), a chemical chaperone having a positive influence on recombinant protein production in CHO cells, were investigated. By varying the timing of 4-PBA addition, it was confirmed that the titer could be increased by 1.57-fold while reducing negative effects such as growth inhibition and apoptosis, thus preserving product quality. These effects of 4-PBA demonstrate the potential of using additive for developing novel enhancers for the production of recombinant proteins and the development of strategies for CHO cell engineering. Chinese hamster ovary (CHO) cells have been widely used in the biotechnology industry for the production of therapeutic proteins, leading to various research efforts to increase the productivity of therapeutic proteins. Although the addition of small molecule enhancers in CHO cell culture can enhance productivity, it poses a problem by negatively affecting product quality. In this study, effects of 4-phenylbutyrate (4-PBA), a chemical chaperone having a positive influence on recombinant protein production in CHO cells, were investigated. By varying the timing of 4-PBA addition, it was confirmed that the titer could be increased by 1.57-fold while reducing negative effects such as growth inhibition and apoptosis, thus preserving product quality. These effects of 4-PBA demonstrate the potential of using additive for developing novel enhancers for the production of recombinant proteins and the development of strategies for CHO cell engineering. KCI Citation Count: 0 |
| Author | Lim, Jungae Cheon, Su-Hwan Lee, Ji-Hoon Kim, Z-Hun Lee, Guewha Kim, Dong-Il Lim, Jin-Hyuk |
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| SubjectTerms | Acids Antibodies Apoptosis Bioengineering Biotechnology Biotechnology industry Cell culture Cell cycle Cell death Cell fusion Chemistry Chemistry and Materials Science Cricetulus griseus Development strategies Engineering Fc receptors Fusion protein growth retardation Industrial and Production Engineering industry Ovaries Phenylbutyric acid Product quality Productivity Protein folding protein synthesis Proteins recombinant proteins Research Paper therapeutics 생물공학 |
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| Title | Effect of 4-phenylbutyrate addition timing on titer of Fc-fusion protein in Chinese hamster ovary cell cultures |
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