Differential translocation of protein kinase C epsilon during HeLa cell adhesion to a gelatin substratum

• Published in: The Journal of biological chemistry Vol. 271; no. 22; pp. 13008 - 13012
• Main Authors: Chun, J S, Ha, M J, Jacobson, B S
• Format: Journal Article
• Language: English
• Published: United States 31.05.1996
• Subjects: Biological Transport; Cell Adhesion; Cell Membrane - enzymology; Cytosol - enzymology; Enzyme Activation; Gelatin - metabolism; HeLa Cells; Humans; Isoenzymes - metabolism; Protein Kinase C - metabolism; Protein Kinase C-epsilon; Tetradecanoylphorbol Acetate - pharmacology

The spreading of HeLa cells, following attachment to a collagen or gelatin substratum, requires the activation of protein kinase C (PKC). Membrane-bound PKC was previously shown to be activated during cell attachment and in response to the activation of a series of lipid second messengers turned on...