Formation and maturation of phagosomes labeled by magnetic micro particles: an ultrastructural study in Tetrahymena thermophila

• Published in: European journal of protistology Vol. 34; no. 3; pp. 291 - 300
• Main Authors: Baumert, Uwe, Vosskühler, Christian, Tiedtke, Arno
• Format: Journal Article
• Language: English
• Published: Elsevier GmbH 12.10.1998
• Subjects: Bacteria; Digestive cycle; Freeze-fracture; Freshwater; Magnetic isolation of phagosomes; Phagocytosis; TEM; Tetrahymena thermophila; Digestive cycle; TEM; Freeze-fracture; Phagocytosis; Magnetic isolation of phagosomes

Phagosomes of Tetrahymena thermophila were pulse-chase labeled with colloidal magnetic iron-starch particles. These labeled phagosomes are easily purified by magnets in large quantities. They allow us to analyse their biochemical composition which is expected to change stage-specifically during thei...