Microchip-based enzyme-linked immunosorbent assay (microELISA) system with thermal lens detection

• Published in: Lab on a chip Vol. 4; no. 6; pp. 570 - 575
• Main Authors: Sato, Kiichi, Yamanaka, Maho, Hagino, Tomokazu, Tokeshi, Manabu, Kimura, Hiroko, Kitamori, Takehiko
• Format: Journal Article
• Language: English
• Published: England 01.01.2004
• Subjects: Enzyme-Linked Immunosorbent Assay - instrumentation; Enzyme-Linked Immunosorbent Assay - methods; Equipment Design; Equipment Failure Analysis; Flow Injection Analysis - instrumentation; Flow Injection Analysis - methods; Humans; Interferon-gamma - analysis; Lab-On-A-Chip Devices; Lenses; Microchip Analytical Procedures - methods; Miniaturization - methods; Systems Integration; Thermography - instrumentation; Thermography - methods

A microchip-based enzyme-linked immunosorbent assay (microELISA) system was developed and interferon-gamma was successfully determined. The system was composed of a microchip with a Y-shaped microchannel and a dam structure, polystyrene microbeads, and a thermal lens microscope (TLM). All reactions...