Production of viable cloned miniature pigs by aggregation of handmade cloned embryos at the 4-cell stage

• Published in: Reproduction fertility and development Vol. 26; no. 3; pp. 395 - 406
• Main Authors: Siriboon, Chawalit, Tu, Ching-Fu, Kere, Michel, Liu, Ming-Sing, Chang, Hui-Jung, Ho, Lin-Lin, Tai, Miao-En, Fang, Wen-Der, Lo, Neng-Wen, Tseng, Jung-Kai, Ju, Jyh-Cherng
• Format: Journal Article
• Language: English
• Published: Australia CSIRO Publishing 01.01.2014
• Subjects: Analysis of Variance; Animals; apoptosis; Apoptosis - physiology; bcl-2-Associated X Protein - metabolism; blastocyst; Blastocyst Inner Cell Mass - cytology; CDX2 Transcription Factor; Cell Aggregation - physiology; Cloning, Organism - methods; Cloning, Organism - veterinary; DNA Primers - genetics; embryo transfer; Embryo, Mammalian - embryology; Female; gene expression; Gene Expression Profiling; genes; Homeodomain Proteins - metabolism; miniature swine; mothers; Octamer Transcription Factor-3 - metabolism; piglets; Pregnancy; Pregnancy Outcome; Real-Time Polymerase Chain Reaction; Swine; Swine, Miniature - embryology; Trans-Activators - metabolism

The aim of the present study was to improve the quality of handmade cloned porcine embryos by multiple embryo aggregations. Embryos derived from aggregation of three cloned embryos (3×) had a better blastocyst rate than cloned control (1×) embryos (73.6% vs 35.1%, respectively; P<0.05), but did n...