Anti-HER2 scFv Expression in Escherichia coli SHuffle®T7 Express Cells: Effects on Solubility and Biological Activity

• Published in: Molecular biotechnology Vol. 62; no. 1; pp. 18 - 30
• Main Authors: Ahmadzadeh, Maryam, Farshdari, Farzaneh, Nematollahi, Leila, Behdani, Mahdi, Mohit, Elham
• Format: Journal Article
• Language: English
• Published: New York Springer US 01.01.2020; Springer Nature B.V
• Subjects: bioactive properties; Biochemistry; Biological activity; Biological Techniques; Biotechnology; Breast cancer; breast neoplasms; Cell Biology; Chemical bonds; Chemistry; Chemistry and Materials Science; Cytoplasm; disulfide bonds; E coli; enzyme-linked immunosorbent assay; Epidermal growth factor; Epitopes; ErbB-2 protein; erbB-2 receptor; Escherichia coli; Flow cytometry; Growth factors; Human Genetics; Inclusion bodies; Medical prognosis; Monoclonal antibodies; Original Paper; prognosis; Protein Science; Solubility; Targeted cancer therapy; Trastuzumab; Tumors; strain; scFv; Solubility; Breast cancer; SHuffle; HER2; Biological activity; SHuffle® strain

Breast cancer is the second most commonly diagnosed cancer, worldwide. Human epidermal growth factor receptor 2 (HER2)-overexpressing breast cancer is correlated with poor prognosis. HER2-targeting monoclonal antibodies resulted in longer survival of HER2 + breast cancer. Single-chain variable fragm...