Efficacy of common hospital biocides with biofilms of multi-drug resistant clinical isolates

Strathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, Glasgow, UK Correspondence Iain S. Hunter i.s.hunter{at}strath.ac.uk Received 30 September 2007 Accepted 26 March 2008 The hospital environment is particularly susceptible to contamination by bacterial pathogens th...

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Published inJournal of medical microbiology Vol. 57; no. 8; pp. 966 - 973
Main Authors Smith, Karen, Hunter, Iain S
Format Journal Article
LanguageEnglish
Published Reading Soc General Microbiol 01.08.2008
Society for General Microbiology
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Abstract Strathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, Glasgow, UK Correspondence Iain S. Hunter i.s.hunter{at}strath.ac.uk Received 30 September 2007 Accepted 26 March 2008 The hospital environment is particularly susceptible to contamination by bacterial pathogens that grow on surfaces in biofilms. The effects of hospital biocides on two nosocomial pathogens, meticillin-resistant Staphylococcus aureus (MRSA) and Pseudomonas aeruginosa , growing as free-floating (planktonic) and adherent biofilm populations (sessile) were examined. Clinical isolates of MRSA and P. aeruginosa were grown as biofilms on discs of materials found in the hospital environment (stainless steel, glass, polyethylene and Teflon) and treated with three commonly used hospital biocides containing benzalkonium chloride (1 % w/v), chlorhexidine gluconate (4 % w/v) and triclosan (1 % w/v). Cell viability following biocide treatment was determined using an XTT assay and the LIVE/DEAD BacLight Bacterial Viability kit. The minimum bactericidal concentration (MBC) of all biocides for planktonic populations of both organisms was considerably less than the concentration recommended for use by the manufacturer. However, when isolates were grown as biofilms, the biocides were ineffective at killing bacteria at the concentrations recommended for use. Following biocide treatment, 0–11 % of cells in MRSA biofilms survived, and up to 80 % of cells in P. aeruginosa biofilms survived. This study suggests that although biocides may be effective against planktonic populations of bacteria, some biocides currently used in hospitals are ineffective against nosocomial pathogens growing as biofilms attached to surfaces and fail to control this reservoir for hospital-acquired infection. Abbreviations: CF, cystic fibrosis; HA-MRSA, hospital-acquired MRSA; MBC, minimum bactericidal concentration; MRSA, meticillin-resistant Staphylococcus aureus ; SEM, scanning electron microscopy. Present address: Department of Biological and Biomedical Sciences, Glasgow Caledonian University, Cowcaddens Road, Glasgow, UK.
AbstractList The hospital environment is particularly susceptible to contamination by bacterial pathogens that grow on surfaces in biofilms. The effects of hospital biocides on two nosocomial pathogens, meticillin-resistant Staphylococcus aureus (MRSA) and Pseudomonas aeruginosa, growing as free-floating (planktonic) and adherent biofilm populations (sessile) were examined. Clinical isolates of MRSA and P. aeruginosa were grown as biofilms on discs of materials found in the hospital environment (stainless steel, glass, polyethylene and Teflon) and treated with three commonly used hospital biocides containing benzalkonium chloride (1% w/v), chlorhexidine gluconate (4% w/v) and triclosan (1 % w/v). Cell viability following biocide treatment was determined using an XTT assay and the LIVE/DEAD BacLight Bacterial Viability kit. The minimum bactericidal concentration (MBC) of all biocides for planktonic populations of both organisms was considerably less than the concentration recommended for use by the manufacturer. However, when isolates were grown as biofilms, the biocides were ineffective at killing bacteria at the concentrations recommended for use. Following biocide treatment, 0-11% of cells in MRSA biofilms survived, and up to 80% of cells in P. aeruginosa biofilms survived. This study suggests that although biocides may be effective against planktonic populations of bacteria, some biocides currently used in hospitals are ineffective against nosocomial pathogens growing as biofilms attached to surfaces and fail to control this reservoir for hospital-acquired infection.
Strathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, Glasgow, UK Correspondence Iain S. Hunter i.s.hunter{at}strath.ac.uk Received 30 September 2007 Accepted 26 March 2008 The hospital environment is particularly susceptible to contamination by bacterial pathogens that grow on surfaces in biofilms. The effects of hospital biocides on two nosocomial pathogens, meticillin-resistant Staphylococcus aureus (MRSA) and Pseudomonas aeruginosa , growing as free-floating (planktonic) and adherent biofilm populations (sessile) were examined. Clinical isolates of MRSA and P. aeruginosa were grown as biofilms on discs of materials found in the hospital environment (stainless steel, glass, polyethylene and Teflon) and treated with three commonly used hospital biocides containing benzalkonium chloride (1 % w/v), chlorhexidine gluconate (4 % w/v) and triclosan (1 % w/v). Cell viability following biocide treatment was determined using an XTT assay and the LIVE/DEAD BacLight Bacterial Viability kit. The minimum bactericidal concentration (MBC) of all biocides for planktonic populations of both organisms was considerably less than the concentration recommended for use by the manufacturer. However, when isolates were grown as biofilms, the biocides were ineffective at killing bacteria at the concentrations recommended for use. Following biocide treatment, 0–11 % of cells in MRSA biofilms survived, and up to 80 % of cells in P. aeruginosa biofilms survived. This study suggests that although biocides may be effective against planktonic populations of bacteria, some biocides currently used in hospitals are ineffective against nosocomial pathogens growing as biofilms attached to surfaces and fail to control this reservoir for hospital-acquired infection. Abbreviations: CF, cystic fibrosis; HA-MRSA, hospital-acquired MRSA; MBC, minimum bactericidal concentration; MRSA, meticillin-resistant Staphylococcus aureus ; SEM, scanning electron microscopy. Present address: Department of Biological and Biomedical Sciences, Glasgow Caledonian University, Cowcaddens Road, Glasgow, UK.
Author Hunter, Iain S
Smith, Karen
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Snippet Strathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, Glasgow, UK Correspondence Iain S. Hunter i.s.hunter{at}strath.ac.uk...
The hospital environment is particularly susceptible to contamination by bacterial pathogens that grow on surfaces in biofilms. The effects of hospital...
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StartPage 966
SubjectTerms Bacteria - drug effects
Bacteria - growth & development
Bacteria - isolation & purification
Bacteria - pathogenicity
Bacterial Infections - epidemiology
Bacterial Infections - prevention & control
Bacterial Infections - transmission
Biofilms
Biological and medical sciences
Cell Survival
Cross Infection - epidemiology
Cross Infection - prevention & control
Disinfectants
Drug Resistance, Multiple
Equipment Design
Fundamental and applied biological sciences. Psychology
Humans
Infectious diseases
Medical sciences
Microbiology
Microscopy, Confocal
Plankton - drug effects
Plankton - growth & development
Plankton - isolation & purification
Polyethylene
Polytetrafluoroethylene
Pseudomonas aeruginosa
Stainless Steel
Staphylococcus aureus
United Kingdom - epidemiology
Title Efficacy of common hospital biocides with biofilms of multi-drug resistant clinical isolates
URI http://jmm.sgmjournals.org/cgi/content/abstract/57/8/966
https://www.ncbi.nlm.nih.gov/pubmed/18628497
https://search.proquest.com/docview/20939977
https://search.proquest.com/docview/69316856
Volume 57
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