A novel ratiometric electrochemical biosensing strategy based on T7 exonuclease-assisted homogenous target recycling coupling hairpin assembly-triggered double-signal output for the multiple amplified detection of miRNA

• Published in: Analyst (London) Vol. 146; no. 8; pp. 275 - 2711
• Main Authors: Zhou, Qing-Yun, Ma, Rong-Na, Hu, Chao-Long, Sun, Fei, Jia, Li-Ping, Zhang, Wei, Shang, Lei, Xue, Qing-Wang, Jia, Wen-Li, Wang, Huai-Sheng
• Format: Journal Article
• Language: English
• Published: England Royal Society of Chemistry 26.04.2021
• Subjects: Amplification; Assembly; Biosensing Techniques; Coupling; Electrochemical Techniques; Exodeoxyribonucleases; Limit of Detection; MicroRNAs; MicroRNAs - genetics; Recycling; Reproducibility of Results; Ribonucleic acid; RNA; Signal processing; Signal transduction

A novel ratiometric electrochemical biosensing strategy based on T7 exonuclease (T7 Exo)-assisted homogenous target recycling coupling hairpin assembly triggered dual-signal output was proposed for the accurate and sensitive detection of microRNA-141 (miRNA-141). Concretely, in the presence of targe...