A novel ratiometric electrochemical biosensing strategy based on T7 exonuclease-assisted homogenous target recycling coupling hairpin assembly-triggered double-signal output for the multiple amplified detection of miRNA

A novel ratiometric electrochemical biosensing strategy based on T7 exonuclease (T7 Exo)-assisted homogenous target recycling coupling hairpin assembly triggered dual-signal output was proposed for the accurate and sensitive detection of microRNA-141 (miRNA-141). Concretely, in the presence of targe...

Full description

Saved in:
Bibliographic Details
Published inAnalyst (London) Vol. 146; no. 8; pp. 275 - 2711
Main Authors Zhou, Qing-Yun, Ma, Rong-Na, Hu, Chao-Long, Sun, Fei, Jia, Li-Ping, Zhang, Wei, Shang, Lei, Xue, Qing-Wang, Jia, Wen-Li, Wang, Huai-Sheng
Format Journal Article
LanguageEnglish
Published England Royal Society of Chemistry 26.04.2021
Subjects
Online AccessGet full text

Cover

Loading…
Abstract A novel ratiometric electrochemical biosensing strategy based on T7 exonuclease (T7 Exo)-assisted homogenous target recycling coupling hairpin assembly triggered dual-signal output was proposed for the accurate and sensitive detection of microRNA-141 (miRNA-141). Concretely, in the presence of target miRNA, abundant signal transduction probes were released via the T7 Exo-assisted homogenous target recycling amplification, which could be captured by the specially designed ferrocene-labeled hairpin probe (Fc-H1) on -electrode interface and triggered the nonenzymatic catalytic hairpin assembly (Fc-H1 + MB-H2) to realize the cascade signal amplification and dual-signal output. Through such a conformational change process, the electrochemical signal of Fc ( I Fc ) and MB ( I MB ) is proportionally and substantially decreased and increased. Therefore, the signal ratio of I MB / I Fc can be employed to accurately reflect the true level of original miRNA. Benefiting from the efficient integration of the T7 Exo-assisted target recycle, nonenzymatic hairpin assembly and dual-signal output mode, the proposed sensor could realize the amplified detection of miRNA-141 effectively with a wide detection range from 1 fM to 100 pM, and a detection limit of 200 aM. Furthermore, it exhibits outstanding sequence specificity to discriminate mismatched RNA, acceptable reproducibility and feasibility for real sample. This strategy effectively integrated the advantages of multiple amplification and ratiometric output modes, which could provide an accurate and efficient method in biosensing and clinical diagnosis. A novel ratiometric electrochemical biosensing strategy based on T7 exonuclease-assisted homogenous target recycling coupling hairpin assembly-triggered dual-signal output was designed for the accurate and sensitive detection of microRNA-141.
AbstractList A novel ratiometric electrochemical biosensing strategy based on T7 exonuclease (T7 Exo)-assisted homogenous target recycling coupling hairpin assembly triggered dual-signal output was proposed for the accurate and sensitive detection of microRNA-141 (miRNA-141). Concretely, in the presence of target miRNA, abundant signal transduction probes were released via the T7 Exo-assisted homogenous target recycling amplification, which could be captured by the specially designed ferrocene-labeled hairpin probe (Fc-H1) on -electrode interface and triggered the nonenzymatic catalytic hairpin assembly (Fc-H1 + MB-H2) to realize the cascade signal amplification and dual-signal output. Through such a conformational change process, the electrochemical signal of Fc ( I Fc ) and MB ( I MB ) is proportionally and substantially decreased and increased. Therefore, the signal ratio of I MB / I Fc can be employed to accurately reflect the true level of original miRNA. Benefiting from the efficient integration of the T7 Exo-assisted target recycle, nonenzymatic hairpin assembly and dual-signal output mode, the proposed sensor could realize the amplified detection of miRNA-141 effectively with a wide detection range from 1 fM to 100 pM, and a detection limit of 200 aM. Furthermore, it exhibits outstanding sequence specificity to discriminate mismatched RNA, acceptable reproducibility and feasibility for real sample. This strategy effectively integrated the advantages of multiple amplification and ratiometric output modes, which could provide an accurate and efficient method in biosensing and clinical diagnosis.
A novel ratiometric electrochemical biosensing strategy based on T7 exonuclease (T7 Exo)-assisted homogenous target recycling coupling hairpin assembly triggered dual-signal output was proposed for the accurate and sensitive detection of microRNA-141 (miRNA-141). Concretely, in the presence of target miRNA, abundant signal transduction probes were released via the T7 Exo-assisted homogenous target recycling amplification, which could be captured by the specially designed ferrocene-labeled hairpin probe (Fc-H1) on -electrode interface and triggered the nonenzymatic catalytic hairpin assembly (Fc-H1 + MB-H2) to realize the cascade signal amplification and dual-signal output. Through such a conformational change process, the electrochemical signal of Fc (IFc) and MB (IMB) is proportionally and substantially decreased and increased. Therefore, the signal ratio of IMB/IFc can be employed to accurately reflect the true level of original miRNA. Benefiting from the efficient integration of the T7 Exo-assisted target recycle, nonenzymatic hairpin assembly and dual-signal output mode, the proposed sensor could realize the amplified detection of miRNA-141 effectively with a wide detection range from 1 fM to 100 pM, and a detection limit of 200 aM. Furthermore, it exhibits outstanding sequence specificity to discriminate mismatched RNA, acceptable reproducibility and feasibility for real sample. This strategy effectively integrated the advantages of multiple amplification and ratiometric output modes, which could provide an accurate and efficient method in biosensing and clinical diagnosis.
A novel ratiometric electrochemical biosensing strategy based on T7 exonuclease (T7 Exo)-assisted homogenous target recycling coupling hairpin assembly triggered dual-signal output was proposed for the accurate and sensitive detection of microRNA-141 (miRNA-141). Concretely, in the presence of target miRNA, abundant signal transduction probes were released via the T7 Exo-assisted homogenous target recycling amplification, which could be captured by the specially designed ferrocene-labeled hairpin probe (Fc-H1) on -electrode interface and triggered the nonenzymatic catalytic hairpin assembly (Fc-H1 + MB-H2) to realize the cascade signal amplification and dual-signal output. Through such a conformational change process, the electrochemical signal of Fc ( I Fc ) and MB ( I MB ) is proportionally and substantially decreased and increased. Therefore, the signal ratio of I MB / I Fc can be employed to accurately reflect the true level of original miRNA. Benefiting from the efficient integration of the T7 Exo-assisted target recycle, nonenzymatic hairpin assembly and dual-signal output mode, the proposed sensor could realize the amplified detection of miRNA-141 effectively with a wide detection range from 1 fM to 100 pM, and a detection limit of 200 aM. Furthermore, it exhibits outstanding sequence specificity to discriminate mismatched RNA, acceptable reproducibility and feasibility for real sample. This strategy effectively integrated the advantages of multiple amplification and ratiometric output modes, which could provide an accurate and efficient method in biosensing and clinical diagnosis. A novel ratiometric electrochemical biosensing strategy based on T7 exonuclease-assisted homogenous target recycling coupling hairpin assembly-triggered dual-signal output was designed for the accurate and sensitive detection of microRNA-141.
Author Sun, Fei
Jia, Li-Ping
Zhang, Wei
Hu, Chao-Long
Xue, Qing-Wang
Jia, Wen-Li
Shang, Lei
Wang, Huai-Sheng
Ma, Rong-Na
Zhou, Qing-Yun
AuthorAffiliation Oncology Department
Hospital of Traditional Chinese Medicine of Jinan City
School of Chemistry and Chemical Engineering
Liaocheng University
AuthorAffiliation_xml – name: Liaocheng University
– name: School of Chemistry and Chemical Engineering
– name: Hospital of Traditional Chinese Medicine of Jinan City
– name: Oncology Department
Author_xml – sequence: 1
  givenname: Qing-Yun
  surname: Zhou
  fullname: Zhou, Qing-Yun
– sequence: 2
  givenname: Rong-Na
  surname: Ma
  fullname: Ma, Rong-Na
– sequence: 3
  givenname: Chao-Long
  surname: Hu
  fullname: Hu, Chao-Long
– sequence: 4
  givenname: Fei
  surname: Sun
  fullname: Sun, Fei
– sequence: 5
  givenname: Li-Ping
  surname: Jia
  fullname: Jia, Li-Ping
– sequence: 6
  givenname: Wei
  surname: Zhang
  fullname: Zhang, Wei
– sequence: 7
  givenname: Lei
  surname: Shang
  fullname: Shang, Lei
– sequence: 8
  givenname: Qing-Wang
  surname: Xue
  fullname: Xue, Qing-Wang
– sequence: 9
  givenname: Wen-Li
  surname: Jia
  fullname: Jia, Wen-Li
– sequence: 10
  givenname: Huai-Sheng
  surname: Wang
  fullname: Wang, Huai-Sheng
BackLink https://www.ncbi.nlm.nih.gov/pubmed/33751013$$D View this record in MEDLINE/PubMed
BookMark eNpdkktv1DAQxy1URLcLF-4gS70gpIAd20n2uCpvVUVC5Rw5ziTrlR_BD8R-Vr4M3m4pEic_5jf_-Y_HF-jMeQcIPafkDSVs83ak0hFSE75_hFaUNbwSou7O0IoQwqq6EfwcXcS4L0dKBHmCzhlrBSWUrdDvLXb-JxgcZNLeQgpaYTCgUvBqB1YrafCgfQQXtZtxTAWE-YAHGWHE3uHbFsMv77IyUK4qGaOOqYR23voZnM8RJxlmSDiAOihzVFE-L3ebndRh0Q6XLLCDOVSl_jxDKPmjz4OBKurZFQs-pyUnPPmA0w6wzSbpxQCWtghN-shDKq51ceQnbPW3m-1T9HiSJsKz-3WNvn94f3v1qbr--vHz1fa6UuUdUgVkUEOrmpqzTgy8oXJsgLbt1NJmpGzsyNRy3irJN4KpTvKaDXQU3SQIEzA1bI1enXSX4H9kiKm3OiowRjoo7fe1IJw1TGxYQS__Q_c-h9LgkaIdEWxTVNfo9YlSwccYYOqXoK0Mh56S_jjy_h3d3tyN_EuBX95L5sHC-ID-nXEBXpyAENVD9N-fYX8Aj2K36A
CitedBy_id crossref_primary_10_1039_D1AN01603B
crossref_primary_10_1039_D2TB00815G
crossref_primary_10_1016_j_aca_2023_341876
crossref_primary_10_1016_j_talanta_2021_122735
crossref_primary_10_1016_j_trac_2023_117508
crossref_primary_10_1021_acsami_1c17234
crossref_primary_10_1016_j_ccr_2022_214565
crossref_primary_10_1016_j_cej_2022_136820
crossref_primary_10_1021_acs_analchem_3c02433
crossref_primary_10_1007_s00604_022_05240_6
crossref_primary_10_1016_j_snb_2022_132901
crossref_primary_10_1021_acs_analchem_1c05293
crossref_primary_10_1016_j_jelechem_2023_117667
crossref_primary_10_1016_j_snb_2022_133144
Cites_doi 10.1021/acs.analchem.0c00130
10.1016/j.bios.2015.04.040
10.1021/acs.analchem.7b00892
10.1039/D0CC00856G
10.1126/science.270.5235.467
10.1021/acs.analchem.9b02914
10.1158/0008-5472.CAN-05-0137
10.1016/j.bios.2016.10.089
10.1021/jacs.7b12772
10.1016/j.snb.2017.10.158
10.1126/science.1091903
10.1158/0008-5472.CAN-07-1083
10.1126/science.1205527
10.1016/j.bios.2017.11.025
10.1021/acs.analchem.8b02523
10.1016/j.bios.2017.01.011
10.1039/C8SC05240A
10.1016/j.coelec.2019.04.014
10.1038/ng1725
10.1261/rna.2332406
10.1021/am502921z
10.1021/acssensors.8b00942
10.1021/ac900598d
10.1007/978-1-60761-944-4_8
10.1186/1479-5876-10-228
10.1021/acs.analchem.8b03425
10.1016/j.bios.2017.11.030
10.1021/acs.analchem.0c02752
10.1016/j.bios.2018.09.028
10.1021/acs.analchem.8b02686
10.1021/acs.analchem.6b04573
10.1021/acs.analchem.9b00690
10.1021/acs.analchem.9b04585
10.1021/acs.analchem.9b02476
10.1126/science.1065329
10.1021/acs.analchem.8b01966
ContentType Journal Article
Copyright Copyright Royal Society of Chemistry 2021
Copyright_xml – notice: Copyright Royal Society of Chemistry 2021
DBID CGR
CUY
CVF
ECM
EIF
NPM
AAYXX
CITATION
7SR
7U5
8BQ
8FD
JG9
L7M
7X8
DOI 10.1039/d1an00204j
DatabaseName Medline
MEDLINE
MEDLINE (Ovid)
MEDLINE
MEDLINE
PubMed
CrossRef
Engineered Materials Abstracts
Solid State and Superconductivity Abstracts
METADEX
Technology Research Database
Materials Research Database
Advanced Technologies Database with Aerospace
MEDLINE - Academic
DatabaseTitle MEDLINE
Medline Complete
MEDLINE with Full Text
PubMed
MEDLINE (Ovid)
CrossRef
Materials Research Database
Engineered Materials Abstracts
Solid State and Superconductivity Abstracts
Technology Research Database
Advanced Technologies Database with Aerospace
METADEX
MEDLINE - Academic
DatabaseTitleList CrossRef
MEDLINE

Materials Research Database
Database_xml – sequence: 1
  dbid: NPM
  name: PubMed
  url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed
  sourceTypes: Index Database
– sequence: 2
  dbid: EIF
  name: MEDLINE
  url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search
  sourceTypes: Index Database
DeliveryMethod fulltext_linktorsrc
Discipline Chemistry
EISSN 1364-5528
EndPage 2711
ExternalDocumentID 10_1039_D1AN00204J
33751013
d1an00204j
Genre Journal Article
GroupedDBID -
0-7
02
0R
1TJ
23M
4.4
5RE
70
705
7~J
AAEMU
AAGNR
AAIWI
AANOJ
AAXPP
ABASK
ABDVN
ABFLS
ABGFH
ABOCM
ABPTK
ABRYZ
ACGFS
ACHRU
ACIWK
ACLDK
ADMRA
ADSRN
AENEX
AFVBQ
AGRSR
AGSTE
AGSWI
ALMA_UNASSIGNED_HOLDINGS
ANUXI
ASKNT
AUDPV
AZFZN
BLAPV
BSQNT
C6K
CKLOX
CS3
EBS
ECGLT
EE0
EF-
F5P
GNO
HR
HZ
H~N
IDZ
J3I
JG
M4U
N9A
O9-
P2P
R7B
R7E
RCNCU
RIG
RPMJG
RRA
RRC
RSCEA
SKM
SKR
SKZ
SLC
SLF
TN5
UPT
VH6
WH7
X
---
-JG
-~X
.HR
0R~
2WC
70~
AAJAE
AAWGC
AAXHV
ABEMK
ABJNI
ABPDG
ABXOH
AEFDR
AENGV
AESAV
AETIL
AFLYV
AFOGI
AGEGJ
AHGCF
APEMP
CGR
COF
CUY
CVF
ECM
EIF
GGIMP
H13
HZ~
NPM
RAOCF
~02
AAYXX
CITATION
7SR
7U5
8BQ
8FD
JG9
L7M
7X8
ID FETCH-LOGICAL-c337t-e0bcb7c624385b461ad6e177f716d13d80f7447ca4953c8a423b1d58f5035ef63
ISSN 0003-2654
IngestDate Wed Dec 04 05:47:39 EST 2024
Thu Oct 10 17:15:34 EDT 2024
Fri Dec 06 01:08:03 EST 2024
Sat Sep 28 08:25:39 EDT 2024
Fri Apr 15 11:43:17 EDT 2022
IsPeerReviewed true
IsScholarly true
Issue 8
Language English
LinkModel OpenURL
MergedId FETCHMERGED-LOGICAL-c337t-e0bcb7c624385b461ad6e177f716d13d80f7447ca4953c8a423b1d58f5035ef63
Notes Electronic supplementary information (ESI) available: Supplemental experimental section and tables. See DOI
10.1039/d1an00204j
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ORCID 0000-0003-3566-8978
0000-0003-0778-2220
0000-0002-5202-689X
PMID 33751013
PQID 2518053950
PQPubID 2047505
PageCount 7
ParticipantIDs proquest_journals_2518053950
crossref_primary_10_1039_D1AN00204J
rsc_primary_d1an00204j
pubmed_primary_33751013
proquest_miscellaneous_2504363593
PublicationCentury 2000
PublicationDate 20210426
PublicationDateYYYYMMDD 2021-04-26
PublicationDate_xml – month: 4
  year: 2021
  text: 20210426
  day: 26
PublicationDecade 2020
PublicationPlace England
PublicationPlace_xml – name: England
– name: London
PublicationTitle Analyst (London)
PublicationTitleAlternate Analyst
PublicationYear 2021
Publisher Royal Society of Chemistry
Publisher_xml – name: Royal Society of Chemistry
References Zhang (D1AN00204J-(cit7)/*[position()=1]) 2018; 140
Miao (D1AN00204J-(cit16)/*[position()=1]) 2018; 90
Schena (D1AN00204J-(cit15)/*[position()=1]) 1995; 270
Xu (D1AN00204J-(cit29)/*[position()=1]) 2017; 89
Chen (D1AN00204J-(cit4)/*[position()=1]) 2004; 33
Jin (D1AN00204J-(cit31)/*[position()=1]) 2017; 91
Gao (D1AN00204J-(cit34)/*[position()=1]) 2015; 71
Wang (D1AN00204J-(cit27)/*[position()=1]) 2020; 92
Xie (D1AN00204J-(cit6)/*[position()=1]) 2011; 333
Castoldi (D1AN00204J-(cit14)/*[position()=1]) 2006; 12
Chen (D1AN00204J-(cit5)/*[position()=1]) 2012; 10
Xu (D1AN00204J-(cit20)/*[position()=1]) 2019; 91
Xu (D1AN00204J-(cit24)/*[position()=1]) 2019; 91
Ma (D1AN00204J-(cit35)/*[position()=1]) 2018; 257
Cui (D1AN00204J-(cit33)/*[position()=1]) 2018; 102
Hou (D1AN00204J-(cit25)/*[position()=1]) 2018; 90
Wen (D1AN00204J-(cit28)/*[position()=1]) 2019; 91
Li (D1AN00204J-(cit38)/*[position()=1]) 2019; 91
Chen (D1AN00204J-(cit1)/*[position()=1]) 2006; 38
Li (D1AN00204J-(cit13)/*[position()=1]) 2009; 81
Zhu (D1AN00204J-(cit32)/*[position()=1]) 2019; 17
Liu (D1AN00204J-(cit17)/*[position()=1]) 2014; 6
Chan (D1AN00204J-(cit3)/*[position()=1]) 2005; 65
Li (D1AN00204J-(cit21)/*[position()=1]) 2020; 92
Gao (D1AN00204J-(cit30)/*[position()=1]) 2017; 89
Wang (D1AN00204J-(cit36)/*[position()=1]) 2017; 92
Johnson (D1AN00204J-(cit2)/*[position()=1]) 2007; 67
Zhang (D1AN00204J-(cit23)/*[position()=1]) 2018; 90
Zhang (D1AN00204J-(cit10)/*[position()=1]) 2020; 56
Chen (D1AN00204J-(cit12)/*[position()=1]) 2011; 687
Wang (D1AN00204J-(cit26)/*[position()=1]) 2018; 122
Nie (D1AN00204J-(cit18)/*[position()=1]) 2018; 90
Yuan (D1AN00204J-(cit37)/*[position()=1]) 2018; 102
Azzouzi (D1AN00204J-(cit22)/*[position()=1]) 2019; 4
Lee (D1AN00204J-(cit11)/*[position()=1]) 2001; 294
Xu (D1AN00204J-(cit9)/*[position()=1]) 2019; 91
Xia (D1AN00204J-(cit19)/*[position()=1]) 2020; 92
Li (D1AN00204J-(cit8)/*[position()=1]) 2019; 10
References_xml – volume: 92
  start-page: 12145
  year: 2020
  ident: D1AN00204J-(cit27)/*[position()=1]
  publication-title: Anal. Chem.
  doi: 10.1021/acs.analchem.0c00130
  contributor:
    fullname: Wang
– volume: 71
  start-page: 158
  year: 2015
  ident: D1AN00204J-(cit34)/*[position()=1]
  publication-title: Biosens. Bioelectron.
  doi: 10.1016/j.bios.2015.04.040
  contributor:
    fullname: Gao
– volume: 89
  start-page: 7077
  year: 2017
  ident: D1AN00204J-(cit29)/*[position()=1]
  publication-title: Anal. Chem.
  doi: 10.1021/acs.analchem.7b00892
  contributor:
    fullname: Xu
– volume: 56
  start-page: 4288
  year: 2020
  ident: D1AN00204J-(cit10)/*[position()=1]
  publication-title: Chem. Commun.
  doi: 10.1039/D0CC00856G
  contributor:
    fullname: Zhang
– volume: 270
  start-page: 467
  year: 1995
  ident: D1AN00204J-(cit15)/*[position()=1]
  publication-title: Science
  doi: 10.1126/science.270.5235.467
  contributor:
    fullname: Schena
– volume: 91
  start-page: 12000
  year: 2019
  ident: D1AN00204J-(cit24)/*[position()=1]
  publication-title: Anal. Chem.
  doi: 10.1021/acs.analchem.9b02914
  contributor:
    fullname: Xu
– volume: 65
  start-page: 6029
  year: 2005
  ident: D1AN00204J-(cit3)/*[position()=1]
  publication-title: Cancer Res.
  doi: 10.1158/0008-5472.CAN-05-0137
  contributor:
    fullname: Chan
– volume: 92
  start-page: 390
  year: 2017
  ident: D1AN00204J-(cit36)/*[position()=1]
  publication-title: Biosens. Bioelectron.
  doi: 10.1016/j.bios.2016.10.089
  contributor:
    fullname: Wang
– volume: 91
  start-page: 12000
  year: 2019
  ident: D1AN00204J-(cit9)/*[position()=1]
  publication-title: Anal. Chem.
  doi: 10.1021/acs.analchem.9b02914
  contributor:
    fullname: Xu
– volume: 140
  start-page: 3988
  year: 2018
  ident: D1AN00204J-(cit7)/*[position()=1]
  publication-title: J. Am. Chem. Soc.
  doi: 10.1021/jacs.7b12772
  contributor:
    fullname: Zhang
– volume: 257
  start-page: 678
  year: 2018
  ident: D1AN00204J-(cit35)/*[position()=1]
  publication-title: Sens. Actuators, B
  doi: 10.1016/j.snb.2017.10.158
  contributor:
    fullname: Ma
– volume: 33
  start-page: 83
  year: 2004
  ident: D1AN00204J-(cit4)/*[position()=1]
  publication-title: Science
  doi: 10.1126/science.1091903
  contributor:
    fullname: Chen
– volume: 67
  start-page: 7713
  year: 2007
  ident: D1AN00204J-(cit2)/*[position()=1]
  publication-title: Cancer Res.
  doi: 10.1158/0008-5472.CAN-07-1083
  contributor:
    fullname: Johnson
– volume: 333
  start-page: 1307
  year: 2011
  ident: D1AN00204J-(cit6)/*[position()=1]
  publication-title: Science
  doi: 10.1126/science.1205527
  contributor:
    fullname: Xie
– volume: 102
  start-page: 87
  year: 2018
  ident: D1AN00204J-(cit33)/*[position()=1]
  publication-title: Biosens. Bioelectron.
  doi: 10.1016/j.bios.2017.11.025
  contributor:
    fullname: Cui
– volume: 90
  start-page: 9591
  year: 2018
  ident: D1AN00204J-(cit25)/*[position()=1]
  publication-title: Anal. Chem.
  doi: 10.1021/acs.analchem.8b02523
  contributor:
    fullname: Hou
– volume: 91
  start-page: 523
  year: 2017
  ident: D1AN00204J-(cit31)/*[position()=1]
  publication-title: Biosens. Bioelectron.
  doi: 10.1016/j.bios.2017.01.011
  contributor:
    fullname: Jin
– volume: 10
  start-page: 5616
  year: 2019
  ident: D1AN00204J-(cit8)/*[position()=1]
  publication-title: Chem. Sci.
  doi: 10.1039/C8SC05240A
  contributor:
    fullname: Li
– volume: 17
  start-page: 47
  year: 2019
  ident: D1AN00204J-(cit32)/*[position()=1]
  publication-title: Curr. Opin. Electrochem.
  doi: 10.1016/j.coelec.2019.04.014
  contributor:
    fullname: Zhu
– volume: 38
  start-page: 228
  year: 2006
  ident: D1AN00204J-(cit1)/*[position()=1]
  publication-title: Nat. Genet.
  doi: 10.1038/ng1725
  contributor:
    fullname: Chen
– volume: 12
  start-page: 913
  year: 2006
  ident: D1AN00204J-(cit14)/*[position()=1]
  publication-title: RNA
  doi: 10.1261/rna.2332406
  contributor:
    fullname: Castoldi
– volume: 6
  start-page: 13034
  year: 2014
  ident: D1AN00204J-(cit17)/*[position()=1]
  publication-title: ACS Appl. Mater. Interfaces
  doi: 10.1021/am502921z
  contributor:
    fullname: Liu
– volume: 4
  start-page: 326
  year: 2019
  ident: D1AN00204J-(cit22)/*[position()=1]
  publication-title: ACS Sens.
  doi: 10.1021/acssensors.8b00942
  contributor:
    fullname: Azzouzi
– volume: 81
  start-page: 5446
  year: 2009
  ident: D1AN00204J-(cit13)/*[position()=1]
  publication-title: Anal. Chem.
  doi: 10.1021/ac900598d
  contributor:
    fullname: Li
– volume: 687
  start-page: 113
  year: 2011
  ident: D1AN00204J-(cit12)/*[position()=1]
  publication-title: Methods Mol. Biol.
  doi: 10.1007/978-1-60761-944-4_8
  contributor:
    fullname: Chen
– volume: 10
  start-page: 228
  year: 2012
  ident: D1AN00204J-(cit5)/*[position()=1]
  publication-title: J. Transl. Med.
  doi: 10.1186/1479-5876-10-228
  contributor:
    fullname: Chen
– volume: 90
  start-page: 11154
  year: 2018
  ident: D1AN00204J-(cit16)/*[position()=1]
  publication-title: Anal. Chem.
  doi: 10.1021/acs.analchem.8b03425
  contributor:
    fullname: Miao
– volume: 102
  start-page: 211
  year: 2018
  ident: D1AN00204J-(cit37)/*[position()=1]
  publication-title: Biosens. Bioelectron.
  doi: 10.1016/j.bios.2017.11.030
  contributor:
    fullname: Yuan
– volume: 92
  start-page: 14550
  year: 2020
  ident: D1AN00204J-(cit19)/*[position()=1]
  publication-title: Anal. Chem.
  doi: 10.1021/acs.analchem.0c02752
  contributor:
    fullname: Xia
– volume: 122
  start-page: 224
  year: 2018
  ident: D1AN00204J-(cit26)/*[position()=1]
  publication-title: Biosens. Bioelectron.
  doi: 10.1016/j.bios.2018.09.028
  contributor:
    fullname: Wang
– volume: 90
  start-page: 12584
  year: 2018
  ident: D1AN00204J-(cit18)/*[position()=1]
  publication-title: Anal. Chem.
  doi: 10.1021/acs.analchem.8b02686
  contributor:
    fullname: Nie
– volume: 89
  start-page: 2464
  year: 2017
  ident: D1AN00204J-(cit30)/*[position()=1]
  publication-title: Anal. Chem.
  doi: 10.1021/acs.analchem.6b04573
  contributor:
    fullname: Gao
– volume: 91
  start-page: 6127
  year: 2019
  ident: D1AN00204J-(cit38)/*[position()=1]
  publication-title: Anal. Chem.
  doi: 10.1021/acs.analchem.9b00690
  contributor:
    fullname: Li
– volume: 92
  start-page: 2074
  year: 2020
  ident: D1AN00204J-(cit21)/*[position()=1]
  publication-title: Anal. Chem.
  doi: 10.1021/acs.analchem.9b04585
  contributor:
    fullname: Li
– volume: 91
  start-page: 12298
  year: 2019
  ident: D1AN00204J-(cit28)/*[position()=1]
  publication-title: Anal. Chem.
  doi: 10.1021/acs.analchem.9b02476
  contributor:
    fullname: Wen
– volume: 294
  start-page: 862
  year: 2001
  ident: D1AN00204J-(cit11)/*[position()=1]
  publication-title: Science
  doi: 10.1126/science.1065329
  contributor:
    fullname: Lee
– volume: 91
  start-page: 12000
  year: 2019
  ident: D1AN00204J-(cit20)/*[position()=1]
  publication-title: Anal. Chem.
  doi: 10.1021/acs.analchem.9b02914
  contributor:
    fullname: Xu
– volume: 90
  start-page: 11892
  year: 2018
  ident: D1AN00204J-(cit23)/*[position()=1]
  publication-title: Anal. Chem.
  doi: 10.1021/acs.analchem.8b01966
  contributor:
    fullname: Zhang
SSID ssj0001050
Score 2.4694734
Snippet A novel ratiometric electrochemical biosensing strategy based on T7 exonuclease (T7 Exo)-assisted homogenous target recycling coupling hairpin assembly...
SourceID proquest
crossref
pubmed
rsc
SourceType Aggregation Database
Index Database
Publisher
StartPage 275
SubjectTerms Amplification
Assembly
Biosensing Techniques
Coupling
Electrochemical Techniques
Exodeoxyribonucleases
Limit of Detection
MicroRNAs
MicroRNAs - genetics
Recycling
Reproducibility of Results
Ribonucleic acid
RNA
Signal processing
Signal transduction
Title A novel ratiometric electrochemical biosensing strategy based on T7 exonuclease-assisted homogenous target recycling coupling hairpin assembly-triggered double-signal output for the multiple amplified detection of miRNA
URI https://www.ncbi.nlm.nih.gov/pubmed/33751013
https://www.proquest.com/docview/2518053950
https://search.proquest.com/docview/2504363593
Volume 146
hasFullText 1
inHoldings 1
isFullTextHit
isPrint
link http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnZ3Pb9MwFMetsR3ggvg1KAxkBLfII6mdOD1WY2hMrBKokwaXynGcrahNqi1BjH-Vv4M7z3bsZOsOg0sUOcqvvk_9vo6f30PobZFov8sVYZEShKXDnOjZF1IMw4wnMSsy88HtaJIcHLPDk_hkY-NPL2qpqbNd-evGdSX_Y1VoA7vqVbL_YFl_UWiAfbAvbMHCsL2VjcdBWf1Qi8BYcalrY8mgrWsjXSKAbF5d6CB189nA5IW4DLTryvU0wZQH6mdV6pzG0ERASGur58FZtaza7K02VDyAjvFSLuwK3WZlds7E_Hw1LwM4Sy2zxSWB-5-e6tqfQV412UIRHRyixW5Tr5raBzT6GEaho9kLrYFzVSvptOty_mUy7otmmzil7lcf8Z8vvp1VjcbkMzwR-dp41I-sKK6gdSI6dtsIg4rAhU67CTGr4dW8_w1kGOnpHLvQ3vfrFBpsOupdZbtymjASx-3Sc9fXs6QHddrvuXkY91TAkFsfsOZhQqoTtL6PxhOzrviw86MuduCae_VBj2a6n45m3bl30JbO38g20dZ4f_rxk5cQIHpDV-pRv5bLq0tH77qzryqpteERiKVzV8TGiKXpA3S_HeXgsUX2IdpQ5SN0d88VF3yMfo-xQRf30MXX0MUdutihiw26uCrxlOOb0MUdutiiiz262KGLW3TxOrr4CrrYoosBXQzoYocu9uhijy6uCmzQfYKOP-xP9w5IW2eESEp5TVSYyYzLZMhoGmcsiUSeqIjzgkdJHtE8DQvOGJdCx2LLVMAIJIvyOC3ikMaqSOg22iyrUj1DmOWhkHQkwH4hY5kQkYBBxWgoaSxlFrEBeuNsNlvZdDKzdS4GaMeZc9Z2NxczGIik4DFHcThAr_1hMJue4ROlgt91ZvIRwhBiRAfoqcXA3wZeVftfOLINXPjmPBKluev357d6thfoXvcP3EGb9XmjXoIur7NXLcV_AfoI7GM
link.rule.ids 314,780,784,27924,27925
linkProvider Royal Society of Chemistry
openUrl ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=A+novel+ratiometric+electrochemical+biosensing+strategy+based+on+T7+exonuclease-assisted+homogenous+target+recycling+coupling+hairpin+assembly-triggered+double-signal+output+for+the+multiple+amplified+detection+of+miRNA&rft.jtitle=Analyst+%28London%29&rft.au=Zhou%2C+Qing-Yun&rft.au=Ma%2C+Rong-Na&rft.au=Hu%2C+Chao-Long&rft.au=Sun%2C+Fei&rft.date=2021-04-26&rft.issn=0003-2654&rft.eissn=1364-5528&rft.volume=146&rft.issue=8&rft.spage=2705&rft.epage=2711&rft_id=info:doi/10.1039%2FD1AN00204J&rft.externalDBID=n%2Fa&rft.externalDocID=10_1039_D1AN00204J
thumbnail_l http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=0003-2654&client=summon
thumbnail_m http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=0003-2654&client=summon
thumbnail_s http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=0003-2654&client=summon