Janus nanocarrier powered by bi-enzymatic cascade system for smart delivery

• Published in: Journal of materials chemistry. B, Materials for biology and medicine Vol. 7; no. 3; pp. 4669 - 4676
• Main Authors: Jimenez-Falcao, Sandra, Joga, Natalia, García-Fernández, Alba, Llopis Lorente, Antoni, Torres, Daniel, de Luis, Beatriz, Sancenón, Félix, Martínez-Ruiz, Paloma, Martínez-Máñez, Ramón, Villalonga, Reynaldo
• Format: Journal Article
• Language: English
• Published: England Royal Society of Chemistry 31.07.2019
• Subjects: Benzimidazoles; Cascade control; Cyclodextrins; Disruption; Doxorubicin; Gluconic acid; Glucose; Glucose oxidase; Gold; Invertase; Nanoparticles; Nanotechnology devices; pH effects; Silica; Silicon dioxide; Sucrose; Sugar; β-Cyclodextrin
• ISSN: 2050-750X; 2050-7518
• DOI: 10.1039/c9tb00938h

We report herein the assembly of an integrated nanodevice with bi-enzymatic cascade control for on-command cargo release. This nanocarrier is based on Au-mesoporous silica Janus nanoparticles capped at the mesoporous face with benzimidazole/β-cyclodextrin-glucose oxidase pH-sensitive gate-like ensembles and functionalized with invertase on the gold face. The rationale for this delivery mechanism is based on the invertase-mediated hydrolysis of sucrose yielding glucose, which is further transformed into gluconic acid by glucose oxidase causing the disruption of the pH-sensitive supramolecular gates at the Janus nanoparticles. This enzyme-powered device was successfully employed in the autonomous and on-demand delivery of doxorubicin in HeLa cancer cells. A novel integrated Janus nanodevice with bi-enzymatic cascade control successfully employed for on-command release of doxorubicin is reported here.