High-throughput stem cell-based phenotypic screening through microniches

• Published in: Biomaterials science Vol. 7; no. 8; pp. 3471 - 3479
• Main Authors: Kolb, Laura, Allazetta, Simone, Karlsson, Maria, Girgin, Mehmet, Weber, Wilfried, Lutolf, Matthias P
• Format: Journal Article
• Language: English
• Published: England Royal Society of Chemistry 23.07.2019
• Subjects: Animals; Bacterial Proteins - genetics; Bacterial Proteins - metabolism; Biotechnology; Cell Line; Fluorescence; Gene sequencing; Glycoproteins - genetics; Glycoproteins - metabolism; Growth factors; HEK293 Cells; High-Throughput Screening Assays - instrumentation; High-Throughput Screening Assays - methods; Humans; Immunoglobulin Fc Fragments - genetics; Immunoglobulin Fc Fragments - metabolism; Intercellular Signaling Peptides and Proteins - genetics; Intercellular Signaling Peptides and Proteins - metabolism; Interleukin-4 - genetics; Interleukin-4 - metabolism; Interleukins; Lab-On-A-Chip Devices; Luminescent Proteins - genetics; Luminescent Proteins - metabolism; Microgels; Polymerization; Protein Engineering - methods; Proteins; Recombinant Fusion Proteins - genetics; Recombinant Fusion Proteins - metabolism; Screening; Sequence Analysis, RNA; STAT6 Transcription Factor - genetics; STAT6 Transcription Factor - metabolism; Stem cells; Stem Cells - cytology; Stem Cells - physiology; Tissue engineering; Workflow

As the field of tissue engineering develops, methods for screening combinations of signals for their effects on stem cell behavior are needed. We introduce a microgel-based screening platform for testing combinations of in situ -generated proteins on stem cell fate in ultrahigh-throughput. Compartme...