An early and late cytotoxicity evaluation of lidocaine on human oral mucosa fibroblasts
Local anesthetic drugs are extensively used in dentistry. However, the cytotoxic effects of these pharmaceutical compounds remain unclear. In this work, we have evaluated the cell viability and cell function of human oral mucosa fibroblasts exposed to different concentrations of lidocaine for increa...
Saved in:
Published in | Experimental biology and medicine (Maywood, N.J.) Vol. 239; no. 1; p. 71 |
---|---|
Main Authors | , , , , , , , |
Format | Journal Article |
Language | English |
Published |
England
01.01.2014
|
Subjects | |
Online Access | Get more information |
Cover
Loading…
Abstract | Local anesthetic drugs are extensively used in dentistry. However, the cytotoxic effects of these pharmaceutical compounds remain unclear. In this work, we have evaluated the cell viability and cell function of human oral mucosa fibroblasts exposed to different concentrations of lidocaine for increasing incubation times, using a global screening methods including structural, metabolic and microanalytical analyses. Our results demonstrate that lidocaine is able to alter cell viability and function even at low concentrations and times, although the effect of lidocaine concentration was more important than the incubation time. First, the structural analysis methods revealed that ≥5% concentrations of lidocaine are able to significantly reduce cell viability. Then, the metabolic 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and water-soluble tetrazolium salt (WST-1) assays suggest that concentrations starting from 1% were able to significantly hinder cell physiology. Finally, electron-probe X-ray microanalysis confirmed the deleterious effects of lidocaine and allowed us to demonstrate that these effects are associated to an apoptosis process of cell death. Therefore, care should be taken when lidocaine is clinically used, and the lowest efficient concentrations should always be used. Furthermore, these results suggest that the comprehensive evaluation method used in this work is accurate and efficient for screening of local anesthetics. |
---|---|
AbstractList | Local anesthetic drugs are extensively used in dentistry. However, the cytotoxic effects of these pharmaceutical compounds remain unclear. In this work, we have evaluated the cell viability and cell function of human oral mucosa fibroblasts exposed to different concentrations of lidocaine for increasing incubation times, using a global screening methods including structural, metabolic and microanalytical analyses. Our results demonstrate that lidocaine is able to alter cell viability and function even at low concentrations and times, although the effect of lidocaine concentration was more important than the incubation time. First, the structural analysis methods revealed that ≥5% concentrations of lidocaine are able to significantly reduce cell viability. Then, the metabolic 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and water-soluble tetrazolium salt (WST-1) assays suggest that concentrations starting from 1% were able to significantly hinder cell physiology. Finally, electron-probe X-ray microanalysis confirmed the deleterious effects of lidocaine and allowed us to demonstrate that these effects are associated to an apoptosis process of cell death. Therefore, care should be taken when lidocaine is clinically used, and the lowest efficient concentrations should always be used. Furthermore, these results suggest that the comprehensive evaluation method used in this work is accurate and efficient for screening of local anesthetics. |
Author | García, José Manuel Alfonso-Rodríguez, Camilo Andrés Martín-Piedra, Miguel Ángel Rodríguez, Ismael Ángel Garzón, Ingrid Oliveira, Ana Celeste Alaminos, Miguel Sánchez-Quevedo, María del Carmen |
Author_xml | – sequence: 1 givenname: Ana Celeste surname: Oliveira fullname: Oliveira, Ana Celeste organization: Tissue Engineering Group, Department of Histology, University of Granada, Granada E18012, Spain – sequence: 2 givenname: Ismael Ángel surname: Rodríguez fullname: Rodríguez, Ismael Ángel – sequence: 3 givenname: Ingrid surname: Garzón fullname: Garzón, Ingrid – sequence: 4 givenname: Miguel Ángel surname: Martín-Piedra fullname: Martín-Piedra, Miguel Ángel – sequence: 5 givenname: Camilo Andrés surname: Alfonso-Rodríguez fullname: Alfonso-Rodríguez, Camilo Andrés – sequence: 6 givenname: José Manuel surname: García fullname: García, José Manuel – sequence: 7 givenname: María del Carmen surname: Sánchez-Quevedo fullname: Sánchez-Quevedo, María del Carmen – sequence: 8 givenname: Miguel surname: Alaminos fullname: Alaminos, Miguel |
BackLink | https://www.ncbi.nlm.nih.gov/pubmed/24131541$$D View this record in MEDLINE/PubMed |
BookMark | eNo1j0tLAzEcxIMo9qF3T5IvsJrXbppjKVqFghfFY_nnhZFsUjZZcb-9LeppZn6HYWaBzlNODqEbSu4olfKetrzlkjDKW8KZFGdofkIN75SaoUUpn4TQVrLuEs2YoJy2gs7R-zphB0OcMCSLI1SHzVRzzd_BhDph9wVxhBpywtnjGGw2EJLDx_wx9nCkA0TcjyYXwD7oIesIpZYrdOEhFnf9p0v09vjwunlqdi_b58161xjOZW04M-C6lVpxSijjIJlRYCR0TjAtPLNeW-tP1qvjfKWY02AFGKG889axJbr97T2Mund2fxhCD8O0_3_IfgC7zVTh |
CitedBy_id | crossref_primary_10_3389_fbioe_2022_876734 crossref_primary_10_1186_s12903_024_04213_w crossref_primary_10_17245_jdapm_2020_20_2_63 crossref_primary_10_1213_ANE_0000000000001422 crossref_primary_10_3390_biomedicines12030509 crossref_primary_10_3389_fmicb_2015_00871 crossref_primary_10_1007_s12630_019_01408_6 crossref_primary_10_1016_j_msec_2016_03_049 crossref_primary_10_1111_jphp_12552 crossref_primary_10_3390_ijms22041923 crossref_primary_10_1371_journal_pone_0129419 crossref_primary_10_1016_j_ejpb_2020_01_007 crossref_primary_10_1166_jbn_2022_3223 |
ContentType | Journal Article |
DBID | CGR CUY CVF ECM EIF NPM |
DOI | 10.1177/1535370213503274 |
DatabaseName | Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed |
DatabaseTitle | MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) |
DatabaseTitleList | MEDLINE |
Database_xml | – sequence: 1 dbid: NPM name: PubMed url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 2 dbid: EIF name: MEDLINE url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search sourceTypes: Index Database |
DeliveryMethod | no_fulltext_linktorsrc |
Discipline | Medicine Anatomy & Physiology Biology |
EISSN | 1535-3699 |
ExternalDocumentID | 24131541 |
Genre | Clinical Trial Research Support, Non-U.S. Gov't Journal Article |
GroupedDBID | --- -~X .55 .GJ 0R~ 2WC 35A 3O- 4.4 53G 5GY 5I- 5I. 9T4 AACMV AATBZ ABJNI ABWRX ACARO ACFIC ACGFS ACGZU ACNCT ACPTO ACSBE ADBBV ADFPL ADWAY AEMJX AENEX AEWDL AEWLI AFIEG AFKRG AFNTS AFOSN AGCDD AHOKE AI. AIIQI AIOMO AJUZI ALMA_UNASSIGNED_HOLDINGS AOIJS ARTOV AYAKG C1A C45 CGR CS3 CUY CVF DC. DU5 EBS ECM EIF EJD EMOBN F5P GROUPED_DOAJ H13 HYE HZ~ H~9 J8X L7B M4V MV1 MVM NPM O9- OK1 OVD P.B P.C RPM RSE SCNPE SFC TRM UPT VH1 W8F WOQ X7M XOL YKV ZCA ZGI ~KM |
ID | FETCH-LOGICAL-c337t-32cae6898310123a72c9ac7a6e42b4f2dfbddf2b4ff9001992ebad4ac49fefde2 |
IngestDate | Sat Sep 28 07:52:44 EDT 2024 |
IsPeerReviewed | true |
IsScholarly | true |
Issue | 1 |
Keywords | calcein ethidium X-ray microanalysis Lidocaine MTT WST-1 human oral mucosa fibroblasts |
Language | English |
LinkModel | OpenURL |
MergedId | FETCHMERGED-LOGICAL-c337t-32cae6898310123a72c9ac7a6e42b4f2dfbddf2b4ff9001992ebad4ac49fefde2 |
PMID | 24131541 |
ParticipantIDs | pubmed_primary_24131541 |
PublicationCentury | 2000 |
PublicationDate | 2014-Jan |
PublicationDateYYYYMMDD | 2014-01-01 |
PublicationDate_xml | – month: 01 year: 2014 text: 2014-Jan |
PublicationDecade | 2010 |
PublicationPlace | England |
PublicationPlace_xml | – name: England |
PublicationTitle | Experimental biology and medicine (Maywood, N.J.) |
PublicationTitleAlternate | Exp Biol Med (Maywood) |
PublicationYear | 2014 |
SSID | ssj0015726 |
Score | 2.2048185 |
Snippet | Local anesthetic drugs are extensively used in dentistry. However, the cytotoxic effects of these pharmaceutical compounds remain unclear. In this work, we... |
SourceID | pubmed |
SourceType | Index Database |
StartPage | 71 |
SubjectTerms | Anesthetics, Local - adverse effects Anesthetics, Local - pharmacology Apoptosis - drug effects Cell Survival - drug effects Cells, Cultured Dose-Response Relationship, Drug Drug Evaluation, Preclinical - methods Female Fibroblasts - metabolism Fibroblasts - pathology Humans Lidocaine - adverse effects Lidocaine - pharmacology Male Middle Aged Mouth Mucosa - metabolism Mouth Mucosa - pathology |
Title | An early and late cytotoxicity evaluation of lidocaine on human oral mucosa fibroblasts |
URI | https://www.ncbi.nlm.nih.gov/pubmed/24131541 |
Volume | 239 |
hasFullText | |
inHoldings | 1 |
isFullTextHit | |
isPrint | |
link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1bb9MwFLY6EIgXBBsMNkB-QLxEKY3t3B67CxqTWiG0ib1Nji9TpTaZukyi-5f7RzvHTtqsA3F5iRK7daucT8ffufgcQj5GiU4TZfIwSYtBKHLNQQ_GWZhoZQ1u0UpjRHc0To5OxfFZfNbr3Xaylq7roq9ufnmu5H-kCmMgVzwl-w-SXS4KA3AP8oUrSBiufyXjYRkYV6AYvd9TYI2BWtRVXf2cKCTXq0reSAmnEw37FpJKePat-dzp_BnmrMvAgt1cFcClfWmnpbe-2wGgW7GpDcojRR3JRZu6M-4f9-_5bkGbTubeeVvKYB92uat6Cabvlfah-oOLa-_K_no1k2Ya4GAWtQm3LkFIzm9wdI_7s2rlxbxNxvfFEPw6ZfgNSLX_wdEEVn2wVuPiiETHxWFatRyHPPGtlFq9zXwVpHsA9VrYN3V5uDm48DSuxVOgNjwecOZbBHWwcjlzYMFwI7DL6M-za-W626kNspFmqHLH6D5qwlpxyjqx8s_rfwUrUzdfX7NyHNs5eUGeN2YKHXrMvSQ9U26SLZBgXc0W9BN1icMODJvkyV5793TUgGKL_BiW1GGTAlgoYpN2sUlX2KSVpUtsUnh22KSITeqxSTvYfEVOvxye7B-FTROPUHGe1iFnSpoky7GhHbAkmTKVS5XKxAhWCMu0LbS2eGtztDdyZgqphVQit8Zqw16TR2VVmjeEstzCBzPDlI2EBWYP1gdYO6iFkoESg7dk27-z80tfqeW8fZs7v53ZJc9WiHtHHltQDeY98My6-OBkdwfAvXsG |
link.rule.ids | 786 |
linkProvider | National Library of Medicine |
openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=An+early+and+late+cytotoxicity+evaluation+of+lidocaine+on+human+oral+mucosa+fibroblasts&rft.jtitle=Experimental+biology+and+medicine+%28Maywood%2C+N.J.%29&rft.au=Oliveira%2C+Ana+Celeste&rft.au=Rodr%C3%ADguez%2C+Ismael+%C3%81ngel&rft.au=Garz%C3%B3n%2C+Ingrid&rft.au=Mart%C3%ADn-Piedra%2C+Miguel+%C3%81ngel&rft.date=2014-01-01&rft.eissn=1535-3699&rft.volume=239&rft.issue=1&rft.spage=71&rft_id=info:doi/10.1177%2F1535370213503274&rft_id=info%3Apmid%2F24131541&rft_id=info%3Apmid%2F24131541&rft.externalDocID=24131541 |