Construction of a novel aminofluorene-based ratiometric near-infrared fluorescence probe for detecting carboxylesterase activity in living cells

• Published in: Analytical methods Vol. 16; no. 23; pp. 3641 - 3645
• Main Authors: Li, Jun-Mei, Liu, Yan-Zhao, Lv, Xiao-Fang, Zhou, Ding-Heng, Zhang, Hong, Chen, Yu-Jin, Li, Kun
• Format: Journal Article
• Language: English
• Published: England Royal Society of Chemistry 13.06.2024
• Subjects: Carboxylesterase; Carboxylesterase - analysis; Carboxylesterase - metabolism; Cells (biology); Emission; Emissions; Fluorenes - chemistry; Fluorescence; Fluorescent Dyes - chemistry; Fluorescent indicators; HeLa Cells; Humans; I.R. radiation; Near infrared radiation; Spectrometry, Fluorescence - methods; Spectroscopy, Near-Infrared - methods

Herein, we constructed a novel aminofluorene-based fluorescence probe ( FEN-CE ) for the detection of carboxylesterase (CE) in living cells by a ratiometric near-infrared (NIR) fluorescence signal. FEN-CE with NIR emission (650 nm) could be hydrolyzed specifically by CE and transformed to FENH with...