Non-variable RNA deletion using the CRISPR-Cas9 technique demonstrated improved outcomes in human intestine single-cell RNA sequencing data, even at half sequencing depths

• Published in: Genomics & informatics Vol. 23; no. 1; pp. 14 - 11
• Main Authors: Kim, Dong Jun, Joh, Christine Suh Yun, Jeong, So Young, Kim, Yong Jun, Koh, Seong Joon, Kim, Hyun Je
• Format: Journal Article
• Language: English
• Published: England BioMed Central 20.05.2025
• Subjects: CRISPR-Cas9; Non-variable RNA; Single cell RNA sequencing; Non-variable RNA; CRISPR-Cas9; Single cell RNA sequencing

In single-cell RNA sequencing (scRNA-seq) data, issues related to the high expression of non-variable RNAs often arise due to organ traits or sample quality. Computational methods, such as SoupX (Young (Gigascience 9:giaa151, 2020)), have been used to solve this problem but it may remove biologicall...