Detection of Sarcocystis halieti in muscles of raptors from Lithuania
The genus comprises a diverse group of apicomplexan parasites that infect reptiles, birds, and mammals. They are characterized by the formation of sarcocysts in the muscles of the intermediate host and the development of sporocysts in the intestines of the definitive host. Raptors usually act as def...
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| Published in | Frontiers in veterinary science Vol. 12; p. 1568013 |
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| Main Authors | , , , |
| Format | Journal Article |
| Language | English |
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2025
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| Abstract | The genus
comprises a diverse group of apicomplexan parasites that infect reptiles, birds, and mammals. They are characterized by the formation of sarcocysts in the muscles of the intermediate host and the development of sporocysts in the intestines of the definitive host. Raptors usually act as definitive hosts for numerous
species; however there is a lack of studies on
in the muscles of raptorial birds. Therefore, we aimed to assess infection rates and identify
species in the muscles of raptors in Lithuania.
Muscle samples from 90 raptors (Accipitriformes, Falconiformes, and Strigiformes) were collected throughout Lithuania and analyzed for
spp. Sarcocysts isolated from fresh methylene blue-stained muscle samples were identified using the internal transcribed spacer region 1 sequence genetic marker.
Under the light microscope, sarcocysts were detected in 8.9% (8/90) of the raptors examined. Sarcocysts were found in the leg muscles of common buzzards (
), tawny owls (
), and a long-eared owl (
); neck muscles of a Eurasian goshawk (
), rough-legged buzzard (
), and long-eared owl; and thoracic muscles of a rough-legged buzzard. We observed no sarcocysts in the cardiac muscles. Representatives of one
species,
.
were molecularly identified in seven birds.
This is the first study to report five new intermediate hosts for
.
. Further investigations are needed to assess the possible pathogenicity of
.
in extra-intestinal organs of raptors. |
|---|---|
| AbstractList | The genus Sarcocystis comprises a diverse group of apicomplexan parasites that infect reptiles, birds, and mammals. They are characterized by the formation of sarcocysts in the muscles of the intermediate host and the development of sporocysts in the intestines of the definitive host. Raptors usually act as definitive hosts for numerous Sarcocystis species; however there is a lack of studies on Sarcocystis in the muscles of raptorial birds. Therefore, we aimed to assess infection rates and identify Sarcocystis species in the muscles of raptors in Lithuania.BackgroundThe genus Sarcocystis comprises a diverse group of apicomplexan parasites that infect reptiles, birds, and mammals. They are characterized by the formation of sarcocysts in the muscles of the intermediate host and the development of sporocysts in the intestines of the definitive host. Raptors usually act as definitive hosts for numerous Sarcocystis species; however there is a lack of studies on Sarcocystis in the muscles of raptorial birds. Therefore, we aimed to assess infection rates and identify Sarcocystis species in the muscles of raptors in Lithuania.Muscle samples from 90 raptors (Accipitriformes, Falconiformes, and Strigiformes) were collected throughout Lithuania and analyzed for Sarcocystis spp. Sarcocysts isolated from fresh methylene blue-stained muscle samples were identified using the internal transcribed spacer region 1 sequence genetic marker.MethodsMuscle samples from 90 raptors (Accipitriformes, Falconiformes, and Strigiformes) were collected throughout Lithuania and analyzed for Sarcocystis spp. Sarcocysts isolated from fresh methylene blue-stained muscle samples were identified using the internal transcribed spacer region 1 sequence genetic marker.Under the light microscope, sarcocysts were detected in 8.9% (8/90) of the raptors examined. Sarcocysts were found in the leg muscles of common buzzards (Buteo buteo), tawny owls (Strix aluco), and a long-eared owl (Asio otus); neck muscles of a Eurasian goshawk (Accipiter gentilis), rough-legged buzzard (Buteo lagopus), and long-eared owl; and thoracic muscles of a rough-legged buzzard. We observed no sarcocysts in the cardiac muscles. Representatives of one Sarcocystis species, S. halieti were molecularly identified in seven birds.ResultsUnder the light microscope, sarcocysts were detected in 8.9% (8/90) of the raptors examined. Sarcocysts were found in the leg muscles of common buzzards (Buteo buteo), tawny owls (Strix aluco), and a long-eared owl (Asio otus); neck muscles of a Eurasian goshawk (Accipiter gentilis), rough-legged buzzard (Buteo lagopus), and long-eared owl; and thoracic muscles of a rough-legged buzzard. We observed no sarcocysts in the cardiac muscles. Representatives of one Sarcocystis species, S. halieti were molecularly identified in seven birds.This is the first study to report five new intermediate hosts for S. halieti. Further investigations are needed to assess the possible pathogenicity of S. halieti in extra-intestinal organs of raptors.ConclusionThis is the first study to report five new intermediate hosts for S. halieti. Further investigations are needed to assess the possible pathogenicity of S. halieti in extra-intestinal organs of raptors. The genus comprises a diverse group of apicomplexan parasites that infect reptiles, birds, and mammals. They are characterized by the formation of sarcocysts in the muscles of the intermediate host and the development of sporocysts in the intestines of the definitive host. Raptors usually act as definitive hosts for numerous species; however there is a lack of studies on in the muscles of raptorial birds. Therefore, we aimed to assess infection rates and identify species in the muscles of raptors in Lithuania. Muscle samples from 90 raptors (Accipitriformes, Falconiformes, and Strigiformes) were collected throughout Lithuania and analyzed for spp. Sarcocysts isolated from fresh methylene blue-stained muscle samples were identified using the internal transcribed spacer region 1 sequence genetic marker. Under the light microscope, sarcocysts were detected in 8.9% (8/90) of the raptors examined. Sarcocysts were found in the leg muscles of common buzzards ( ), tawny owls ( ), and a long-eared owl ( ); neck muscles of a Eurasian goshawk ( ), rough-legged buzzard ( ), and long-eared owl; and thoracic muscles of a rough-legged buzzard. We observed no sarcocysts in the cardiac muscles. Representatives of one species, . were molecularly identified in seven birds. This is the first study to report five new intermediate hosts for . . Further investigations are needed to assess the possible pathogenicity of . in extra-intestinal organs of raptors. BackgroundThe genus Sarcocystis comprises a diverse group of apicomplexan parasites that infect reptiles, birds, and mammals. They are characterized by the formation of sarcocysts in the muscles of the intermediate host and the development of sporocysts in the intestines of the definitive host. Raptors usually act as definitive hosts for numerous Sarcocystis species; however there is a lack of studies on Sarcocystis in the muscles of raptorial birds. Therefore, we aimed to assess infection rates and identify Sarcocystis species in the muscles of raptors in Lithuania.MethodsMuscle samples from 90 raptors (Accipitriformes, Falconiformes, and Strigiformes) were collected throughout Lithuania and analyzed for Sarcocystis spp. Sarcocysts isolated from fresh methylene blue-stained muscle samples were identified using the internal transcribed spacer region 1 sequence genetic marker.ResultsUnder the light microscope, sarcocysts were detected in 8.9% (8/90) of the raptors examined. Sarcocysts were found in the leg muscles of common buzzards (Buteo buteo), tawny owls (Strix aluco), and a long-eared owl (Asio otus); neck muscles of a Eurasian goshawk (Accipiter gentilis), rough-legged buzzard (Buteo lagopus), and long-eared owl; and thoracic muscles of a rough-legged buzzard. We observed no sarcocysts in the cardiac muscles. Representatives of one Sarcocystis species, S. halieti were molecularly identified in seven birds.ConclusionThis is the first study to report five new intermediate hosts for S. halieti. Further investigations are needed to assess the possible pathogenicity of S. halieti in extra-intestinal organs of raptors. |
| Author | Butkauskas, Dalius Šukytė, Tautvilė Prakas, Petras Juozaitytė-Ngugu, Evelina |
| AuthorAffiliation | Laboratory of Molecular Ecology, Nature Research Centre , Vilnius , Lithuania |
| AuthorAffiliation_xml | – name: Laboratory of Molecular Ecology, Nature Research Centre , Vilnius , Lithuania |
| Author_xml | – sequence: 1 givenname: Petras surname: Prakas fullname: Prakas, Petras – sequence: 2 givenname: Tautvilė surname: Šukytė fullname: Šukytė, Tautvilė – sequence: 3 givenname: Evelina surname: Juozaitytė-Ngugu fullname: Juozaitytė-Ngugu, Evelina – sequence: 4 givenname: Dalius surname: Butkauskas fullname: Butkauskas, Dalius |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/40761837$$D View this record in MEDLINE/PubMed |
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| ContentType | Journal Article |
| Copyright | Copyright © 2025 Prakas, Šukytė, Juozaitytė-Ngugu and Butkauskas. Copyright © 2025 Prakas, Šukytė, Juozaitytė-Ngugu and Butkauskas. 2025 Prakas, Šukytė, Juozaitytė-Ngugu and Butkauskas |
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| Keywords | methylene blue staining Strigiformes Accipitriformes ITS1 Sarcocystis molecular identification |
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comprises a diverse group of apicomplexan parasites that infect reptiles, birds, and mammals. They are characterized by the formation of sarcocysts... The genus Sarcocystis comprises a diverse group of apicomplexan parasites that infect reptiles, birds, and mammals. They are characterized by the formation of... BackgroundThe genus Sarcocystis comprises a diverse group of apicomplexan parasites that infect reptiles, birds, and mammals. They are characterized by the... |
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| SubjectTerms | Accipitriformes ITS1 methylene blue staining molecular identification Sarcocystis Strigiformes Veterinary Science |
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| Title | Detection of Sarcocystis halieti in muscles of raptors from Lithuania |
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