Scale up in preparative chromatography

• Published in: Journal of Chromatography A Vol. 752; no. 1; pp. 19 - 29
• Main Authors: Heuer, Christian, Hugo, Peter, Mann, Gregor, Seidel-Morgenstern, Andreas
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 01.11.1996; Elsevier
• Subjects: Adsorption isotherms; Analytical chemistry; Chemistry; Chromatographic methods and physical methods associated with chromatography; Exact sciences and technology; Other chromatographic methods; Preparative chromatography; Thermodynamic parameters; Thermodynamic parameters; Preparative chromatography; Adsorption isotherms; Steroid; Separation; Scale effect; Epimer; Adsorption isotherm; Prediction; Liquid chromatography; Experimental study; Optimization; Extrapolation; Optical resolution; Enantiomer; Thermodynamic properties

The subject of the paper is the application of a theoretical concept to scale up preparative chromatography. As an experimental example, the separation of the α- and β-isomers of a steroid compound using silica as the stationary phase is discussed. The applied concept is mainly based on measuring th...