Optimization and validation of singleplex and multiplex RT-qPCR for detection of citrus bark cracking viroid (CBCVd), hop latent viroid (HLVd), and hop stunt viroid (HSVd) in hops (Humulus lupulus L.)

• Published in: Plant disease
• Main Authors: Guček, Tanja, Jakše, Jernej, Radisek, Sebastjan
• Format: Journal Article
• Language: English
• Published: United States 01.11.2023
• Subjects: Pathogen detection; Causal Agent; Viruses and viroids; Field crops; Crop Type; Subject Areas

Direct crop losses due to plant diseases and the measures used to control them have significant agricultural and economic impacts. The shift from diverse small-scale to large-scale genetically uniform monoculture production, along with agricultural intensification and climate change, has led to seve...