Comparison of simple and rapid cell wall disruption methods for improving lipid extraction from yeast cells

• Published in: Journal of microbiological methods Vol. 176; p. 105999
• Main Authors: Kot, Anna M., Gientka, Iwona, Bzducha-Wróbel, Anna, Błażejak, Stanisław, Kurcz, Agnieszka
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 01.09.2020
• Subjects: Acid hydrolysis; Disruption; SCO; Sonication; Yeast lipids; Acid hydrolysis; Sonication; Yeast lipids; Disruption; SCO

The present study examined the effect of six disruption methods of the cell wall (acid hydrolysis, ultrasonication, osmotic shock, pasteurization, homogenization with zirconia balls, and freezing/defrosting) on the efficiency of lipid extraction from yeast cells and the composition of fatty acids. A...