Transformation of Paramecium caudatum with a novel expression vector harboring codon-optimized GFP gene

• Published in: Gene Vol. 284; no. 1-2; pp. 233 - 240
• Main Authors: Takenaka, Yasuhiro, Haga, Nobuyuki, Harumoto, Terue, Matsuura, Tadashi, Mitsui, Youji
• Format: Journal Article
• Language: English
• Published: Netherlands 06.02.2002
• Subjects: Animals; Base Sequence; Cloning, Molecular; Freshwater; Gene Expression; Genetic Vectors - genetics; Green Fluorescent Proteins; Luminescent Proteins - genetics; Luminescent Proteins - metabolism; Microscopy, Fluorescence; Molecular Sequence Data; Paramecium - genetics; Sequence Homology, Nucleic Acid; Transformation, Genetic; Tubulin - genetics

We have developed a novel expression vector, pTub-tel3, for transformation in Paramecium caudatum. The vector was constructed by cloning P. caudatum alpha-tubulin 5' and 3' non-coding regions. To examine transformation with the pTub-tel3 construct, we chose the green fluorescent protein (G...