Lectins are sensitive tools for defining the differentiation programs of mouse gut epithelial cell lineages
We have used histochemical methods to survey the cellular patterns of binding of a panel of 45 lectins with well-defined carbohydrate specificities to sections prepared from various regions of the gastric-to-colonic axis of fetal, neonatal, and adult FVB/N mouse gut. The results suggest that lectins...
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Published in | The American journal of physiology Vol. 266; no. 6 Pt 1; p. G987 |
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Main Authors | , , |
Format | Journal Article |
Language | English |
Published |
United States
01.06.1994
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Abstract | We have used histochemical methods to survey the cellular patterns of binding of a panel of 45 lectins with well-defined carbohydrate specificities to sections prepared from various regions of the gastric-to-colonic axis of fetal, neonatal, and adult FVB/N mouse gut. The results suggest that lectins can be used as remarkably sensitive tools to describe the differentiation programs of gastric and intestinal epithelial cell lineages as a function of their position along the cephalocaudal axis of the gut and as a function of developmental stage. Studies of intestinal isografts and transgenic mice that express Simian virus-40 T antigen in enterocytes suggest that many of these cell lineage-specific and spatial patterns of glycoconjugate production can be established and maintained in the absence of exposure to luminal contents and in the presence of specific proliferative abnormalities. This lectin panel should be useful for operationally defining subpopulations of the principal gut epithelial cell lineages in normal strains of mice, for describing variations in gut epithelial cell differentiation programs in mutant and transgenic mice, and for recovering specific epithelial cell lineages or subpopulations. |
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AbstractList | We have used histochemical methods to survey the cellular patterns of binding of a panel of 45 lectins with well-defined carbohydrate specificities to sections prepared from various regions of the gastric-to-colonic axis of fetal, neonatal, and adult FVB/N mouse gut. The results suggest that lectins can be used as remarkably sensitive tools to describe the differentiation programs of gastric and intestinal epithelial cell lineages as a function of their position along the cephalocaudal axis of the gut and as a function of developmental stage. Studies of intestinal isografts and transgenic mice that express Simian virus-40 T antigen in enterocytes suggest that many of these cell lineage-specific and spatial patterns of glycoconjugate production can be established and maintained in the absence of exposure to luminal contents and in the presence of specific proliferative abnormalities. This lectin panel should be useful for operationally defining subpopulations of the principal gut epithelial cell lineages in normal strains of mice, for describing variations in gut epithelial cell differentiation programs in mutant and transgenic mice, and for recovering specific epithelial cell lineages or subpopulations. |
Author | Roth, K A Falk, P Gordon, J I |
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BackLink | https://www.ncbi.nlm.nih.gov/pubmed/8023947$$D View this record in MEDLINE/PubMed |
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SubjectTerms | Aging - metabolism Aging - physiology Animals Antigens, Viral, Tumor - metabolism Cell Differentiation Cell Line Embryo, Mammalian - cytology Embryo, Mammalian - metabolism Embryonic and Fetal Development Immunohistochemistry Intestinal Mucosa - cytology Intestinal Mucosa - embryology Intestinal Mucosa - growth & development Intestines - cytology Intestines - metabolism Intestines - transplantation Lectins Mice Mice, Inbred Strains Mice, Transgenic Simian virus 40 - metabolism Stomach - cytology Stomach - embryology Stomach - growth & development Transplantation, Isogeneic |
Title | Lectins are sensitive tools for defining the differentiation programs of mouse gut epithelial cell lineages |
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