Diversity in octadecanoid-induced gene expression of tomato

• Published in: Journal of plant physiology Vol. 152; no. 2; pp. 345 - 352
• Main Authors: Wasternack, Claus, Ortel, Birgit, Miersch, Otto, Kramell, Robert, Beale, Michael, Greulich, Friedemann, Feussner, Ivo, Hause, Bettina, Krumm, Thomas, Boland, Wilhelm, Parthier, Benno
• Format: Journal Article
• Language: English
• Published: Jena Elsevier GmbH 1998; Elsevier
• Subjects: 12-oxo-phytodienoic acid; Biological and medical sciences; conjugates; coronatine; diversity of signals; Fundamental and applied biological sciences. Psychology; Generalities. Disease free stocks; Jasmonates; Physical agents; Phytopathology. Animal pests. Plant and forest protection; Plant physiology and development; Vegetative apparatus, growth and morphogenesis. Senescence; ABA; PR; JM; OPC; 12-oxo-phytodienoic acid; pin2; Jasmonates; PDA; (−)IL; AOC; MJIle; HICIM; jrg; Cab-1B; AOS; coronatine; dinor-PDA; (+)ID; Cor; diversity of signals; (−)ID; (+)IL; JIP; conjugates; LOX; JA; Cfa; Terpenoid; Indan derivatives; Injury; Gene expression; Signal transduction; Vegetable crop; Gene; Structure activity relation; Dicotyledones; Angiospermae; Lycopersicon esculentum; Defense mechanism; Spermatophyta; Solanaceae
• ISSN: 0176-1617; 1618-1328
• DOI: 10.1016/S0176-1617(98)80149-1

In tomato plants wounding leads to up-regulation of various plant defense genes via jasmonates ( Ryan, 1992; Bergey et al., 1996). Using this model system of jasmonic acid (JA) signalling, we analyzed activity of octadecanoids to express JA-responsive genes. Leaf treatments were performed with naturally occurring octadecanoids and their molecular mimics such as coronatine or indanone conjugates. JA responses were recorded in terms of up- or down-regulation of various genes by analyzing transcript accumulation, and at least partially in vitro translation products and polypeptide pattern of leaf extracts. The data suggest: (i) 12-Oxo-phytodienoic acid and other intermediates of the octadecanoid pathway has to be ß-oxidized to give a JA response, (ii) Octadecanoids which can not be ß-oxidized are inactive, (iii) JA, its methyl ester (JM), and its amino acid conjugates are most active signals in tomato leaves leading to up regulation of mainly wound-inducible genes and down-regulation of mainly <house-keeping> genes, (iv) Some compounds carrying a JA/JM- or JA amino acid conjugate-like structure induce/repress only a subset of genes suggesting diversity of JA signalling.