Molecular and Cytogenetic Identification of Stem Rust Resistant Wheat-Thinopyrum intermedium Introgression Lines

• Published in: Plant disease
• Main Authors: Li, Jianbo, Bao, Yinguang, Han, Ran, Wang, Xiaolu, Xu, Wenjing, Li, Guangrong, Yang, Zujun, Zhang, Xiaojun, Li, Xin, Liu, Aifeng, Li, Haosheng, Liu, Jianjun, Zhang, Peng, Liu, Cheng
• Format: Journal Article
• Language: English
• Published: United States 01.09.2022
• Subjects: cultivar/resistance; Field crops; Techniques; Crop Type; cereals and grains; Disease management; Subject Areas
• ISSN: 0191-2917
• DOI: 10.1094/PDIS-10-21-2274-RE

Thinopyrum intermedium (JJJsJsStSt, 2n = 6x = 42), a wild relative of common wheat, possesses many desirable agronomic genes for wheat improvement. The production of wheat-Th. intermedium introgression lines is a key step for transferring these beneficial genes into wheat. In this study, we characterized three wheat-Th. intermedium introgression lines TA3681, TA5566, and TA5567 using non-denaturing fluorescence in situ hybridization (ND-FISH), genomic in situ hybridization (GISH), polymerase chain reaction (PCR)-based landmark unique gene (PLUG) and intron targeting (IT) markers. Our results showed that TA3681 is a wheat-Th. intermedium 1St disomic addition line, TA5566 is a wheat-Th. intermedium non-Robertsonian translocation line carrying two pairs of 3A-7Js translocation chromosomes, and TA5567 is a wheat-Th. intermedium non-Robertsonian translocation line carrying a pair of 3A-7Js translocation chromosomes. We developed 13, 36, and 15 Th. intermedium chromosome-specific markers for detecting the introgressed Thinopyrum chromosomes in TA3681, TA5566, and TA5567, respectively. Stem rust assessment revealed that TA3681 exhibited high level of seedling resistance to Chinese prevalent Puccinia graminis f. sp. tritici (Pgt) pathotypes, and both TA5566 and TA5567 were highly resistant to Australian Pgt pathotypes, indicating that Th. intermedium chromosomes 1St and 7Js might carry new stem rust resistance genes. Therefore, the new identified introgression lines may be useful for improving wheat stem rust resistance.